广西医科大学学报2017，Vol.34Issue(6)：816-819,4.DOI:10.16190/j.cnki.45-1211/r.2017.06.005
TGF-β/Smad通路在大鼠放射性肺损伤中的作用
Research on the regulatory mechanism of TGF-β/Smad signal pathway in the radiation-induced lung injury in rats
摘要
Abstract
Objective:To investigate the regulatory mechanism of TGF-β/Smad signal pathway in the radiation-induced lung injury (RILI).Methods:144 male SD rats were randomly divided into six groups:normal control group (A),8 Gy irradiation group (B),10 Gy irradiation group (C),12 Gy irradiation group (D),14 Gy irradiation group (E),16 Gy irradiation group (F),24 mice in each group.The models of RILI in SD rats were established by irradiating with a single dose of 0 Gy (sham),8 Gy,10 Gy,12 Gy,14 Gy,16 Gy over right hemithorax using 60Co 7 therapeutic machine.At 2,5,12 and 24 weeks post-irradiation,five rats from each group were sacrificed.The pathological changes of lung tissue were evaluated by hematoxylin and eosin (HE) stains and Masson stains.Western blotting was performed to detect the protein expression of Smad2 and Smad7.Results:(1) The alveolar structure of lung tissue of group A was intact,and no obvious exudation and hyperplasia were observed.At the end of the 5th and 12th week after irradiation,pathologic changes in the lung tissue of all irradiated rats exhibited chronic inflammatory changes,manifested as lymphocyte infiltration,collagen deposition and fibrous tissue hyperplasia.At week 24 after irradiation,hyperemia and edema subsided in group B,C and D,lymphocyte infiltration was still visible in part of the lung tissue,pulmonary interstitial fibrosis were less compared to week 12.While in the group E and F,the tissue showed thickened significant alveolar wall,incomplete alveolar wall,more lymphocytes infiltration,and significant pulmonary interstitial fibrosis hyperplasia.(2) Compared to group A,the expression of Smad2 in the groups B,C,D,E peaked at 5 weeks,and fell at 12 and 24 weeks (P＜0.05).It also rapidly reached to the peak at 5th weeks also in the 16 Gy irradiation group F,but it did not fall at 12 and 24 weeks and was significant higher than those in other groups (P ＜ 0.05).The expression of Smad7 proteins were also increased at 2 and 5 weeks in all the irradiation group (P ＜0.05).It dropped sharply in groups B,C,D at 12 weeks (all P＜0.05),while it did not fall in groups E and F (all P＞0.05).Conclusion:The TGF-β/Smad signaling pathway plays an important role in the development of the radiation-induced lung injury.关键词
大鼠/放射性肺损伤/TGF-β/Smad信号通路/调控Key words
rats/radiation-induced lung injury/TGF-β/Smad signal pathway/regulate分类
医药卫生引用本文复制引用
欧雪,王寒蕾,李其耕,李路,刘文其..TGF-β/Smad通路在大鼠放射性肺损伤中的作用[J].广西医科大学学报,2017,34(6):816-819,4.基金项目
广西自然科学基金资助项目(No.2015GXNSFAA139192) （No.2015GXNSFAA139192）
