广西医科大学学报2017，Vol.34Issue(6)：820-822,3.DOI:10.16190/j.cnki.45-1211/r.2017.06.006
黄根提取物抗乙型肝炎病毒活性的初步研究
Preliminary research on the anti-HBV activity of Prismatomeris Connata extracts
摘要
Abstract
Objective:To study the effects of Prismatomeris Connata extracts on anti-hepatitis B virus.Methods:A compound was separated and purified from Prismatomeris Connata extracts by silica gel column,thin-layer chromatography and recrystallization.The structure of the compound was analyzed by HPLC,FT-IR,1H NMR and 13C NMR.Using lamivudine (3TC) as positive control drug,the half inhibitory concentration (IC50) of the purified compound on HepG2.2.15 cells was detected by MTT assay and its effect on HBeAg and HBsAg secretions was determined by enzyme-linked immunosorbent assay (ELISA).Results:The purity of the compound isolated from Prismatomneris Connata was greater than 95 ％ by HPLC analysis,and was identified to be 1,3-dihydroxy-2-methyl-9,10-anthraquinone (DMA).The IC50 of DMA on HepG2.2.15 cells was 13.79 mg/L and it remarkably decreased the amounts of secretion HBsAg and HBsAg in a dose manner.DMA (8 mg/L) inhibited HBeAg secretion more effectively than 3TC (100 mg/L) did (P＜0.01).Conclusion:DMA could reduce HBeAg secretion in HepG2.2.15 cell,and it might be a novel anti-HBV drug candidate.关键词
黄根/蒽醌类/抗乙肝病毒Key words
Prismatomeris Connata/anthraquinone/anti-HBV分类
医药卫生引用本文复制引用
彭政,田炜,蓝富,黎丹戎,侯华新..黄根提取物抗乙型肝炎病毒活性的初步研究[J].广西医科大学学报,2017,34(6):820-822,3.基金项目
国家自然科学基金资助项目(No.81460561) （No.81460561）
广西高校重点实验室—壮医方药基础与应用研究重点实验室资助项目(No.桂教研科[2014]6号) （No.桂教研科[2014]6号）
