广西医科大学学报2017，Vol.34Issue(6)：823-827,5.DOI:10.16190/j.cnki.45-1211/r.2017.06.007
miR-23b在哮喘小鼠气道平滑肌中的表达及其功能探讨
Expression and function of miRNA-23b in airway smooth muscle cells from asthmatic mice
张炜 1黄林洁 2陈茗2
作者信息
- 1. 深圳市南山区西丽人民医院呼吸内科,深圳518055
- 2. 广州市中山大学孙逸仙纪念医院呼吸内科,广州 510120
- 折叠
摘要
Abstract
Objective:To investigate the expression change and functions of miR-23b in the airway smooth muscle cells (ASMCs) of asthma mice.Methods:Sixteen female BALB/c mice of clean grade were randomly divided into a normal group and an asthma group.Ovalbu-min (OVA) was used to establish the asthmatic mice model.At 24-48 h after the last irritation,airway smooth muscles were collected and the expression of miR-23b in normal and asthma mice were analyzed via q-PCR.Meanwhile,ASMCs from both normal control group and asthma model group were isolated and cultured by tissue explants adherent method.MiR-23b mimics or mimic NC were transfect into asthma model mice and the AMSCs were collected as well.The proliferation,apoptosis and TGFβR2 expression of the four group ASMCs were detected by CCK8,flow cytometry and western blotting,respectively.Results:Compared with normal mice,the expression of miR-23b was decreased significantly in the airway smooth muscles of asthma mice.Compared with normal control ASMCs,the proliferation of asthma model ASMCs was increased notably (P＜0.05).But after transfected with miR-23b mimics,the proliferation and apoptosis of asthma model ASMCs were decreased compared to asthma model ASMCs.And the expression of TGFβR2 was reduced after transfected with miR-23b mimics (all P＜0.05).Conclusion:The decreased expression of miR-23b might contribute to the over-proliferation of asthma model ASMCs and the decrease of apoptosis,which might be related to the down-regulation of TGFβR2.关键词
miRNA-23b/平滑肌细胞/转化生长因子β受体2/哮喘小鼠Key words
miRNA-23b/airway smooth muscle cells/TGFβR2/asthmatic mice分类
医药卫生引用本文复制引用
张炜,黄林洁,陈茗..miR-23b在哮喘小鼠气道平滑肌中的表达及其功能探讨[J].广西医科大学学报,2017,34(6):823-827,5.
