海南医学院学报2017，Vol.23Issue(8)：1027-1029,1033,4.DOI:10.13210/j.cnki.jhmu.20170406.056
扩张型心肌病动物模型心肌细胞电重构与氧化应激及炎症性损伤的相关性
Correlation between electrical cell remodeling and oxidative stress and inflammatory injury in animal model of dilated cardiomyopathy
摘要
Abstract
Objective: To study the correlation of the electrical remodeling of myocardial cellwith oxidative stresand inflammatory injury in animal modelwith dilated cardiomyopathy.Methods: Male New Zealand white rabbitwere selected aexperimental animaland divided into model group and control group, model group were made into animal modelwith dilated cardiomyopathy through eaintravenouinjection of adriamycin, and the control group received eaintravenouinjection of same dose of saline.Eighweeklater, the myocardial tissue wacollected to detecthe myocardial cell delay aftedepolarization (DADs) through the technique of unicell patch clamp, and radioimmunoprecipitation kitand enzyme-linked immunosorbenassay kitwere used to determine oxidative stresindexeand inflammatory reaction indexes.Results: The incidence of myocardial cell DADof animal model group wasignificantly highethan thaof control group, and ROS, MDA, AOPP, CHOP, GRP78, TLR4, NF-kB, IL-1β, IL-6 and TNF-α levelin myocardial tissue were significantly highethan those of control group;ROS, MDA, AOPP, CHOP, GRP78, TLR4, NF-kB, IL-1β, IL-6 and TNF-α levelin myocardial tissue of DADanimalin model group were significantly highethan those of non-DADanimals.Conclusions: Adriamycin-induced dilated cardiomyopathy can lead to myocardial cell delay aftedepolarization through oxidative stresand inflammatory injury.关键词
阿霉素/扩张型心肌病/晚期后除极/氧化应激反应/炎症反应Key words
Adriamycin/Dilated cardiomyopathy/Delay after depolarization/Oxidative stress response/Inflammatory response分类
医药卫生引用本文复制引用
张易民,张晓辉,王宝娣..扩张型心肌病动物模型心肌细胞电重构与氧化应激及炎症性损伤的相关性[J].海南医学院学报,2017,23(8):1027-1029,1033,4.基金项目
陕西省卫计委(2014D30) （2014D30）
It was supported by Shaanxi Provincial Commission of Health and Family Planning (No.2014D30). （No.2014D30）
