海南医学院学报2017，Vol.23Issue(8)：1034-1036,1040,4.DOI:10.13210/j.cnki.jhmu.20170406.010
舒尼替尼通过Wnt/β-catenin信号抑制肾癌细胞体外生长和侵袭的实验研究
Sunitinib inhibits renal cancer cell growth in vitro and invasion through Wnt/β-catenin signaling pathway: An experimental study
摘要
Abstract
Objective: To study the effecof sunitinion renal cancecell growth in vitro and invasion awell aWnt/β-catenin signaling pathway.Methods: Renal cancecell lineACHN were cultured and processed with differendoseof sunitini(1 μmol/L, 2 μmol/L, 4 μmol/L and 8 μmol/L), and sunitinib-free processing condition waused anegative control.Twenty-fouhourafteprocessing, the mRNexpression levelof apoptosigenes, invasion geneand Wnt/β-catenin signaling pathwayin cellwere detected.Results: Twenty-fouhouraftetreatment, NPRL2, Bax, caspase-3 and caspase-9 mRNexpression in 1 μmol/L, 2 μmol/L, 4 μmol/L and 8 μmol/L sunitinigroupwere significantly highethan those in negative control group while MMP2, MMP9, Vimentin, N-cadherin, Wnand β-catenin mRNexpression were significantly lowethan those in negative control group;the highethe dose of sunitinib, the highethe NPRL2, Bax, caspase-3 and caspase-9 mRNexpression while the lowethe MMP2, MMP9, Vimentin, N-cadherin, Wnand β-catenin mRNexpression in cells.Conclusions: Sunitinican inhibithe Wnt/β-catenin signaling pathway in renal cancecellto increase the expression of apoptosigenes, inhibithe expression of invasion geneand thereby inhibithe cell growth and invasion.关键词
肾癌/舒尼替尼/Wnt/β-catenin/凋亡/侵袭Key words
Renal cancer/Sunitinib/Wnt/β-catenin/Apoptosis/invasion分类
医药卫生引用本文复制引用
杨渝,邵志强,郭嘉祥..舒尼替尼通过Wnt/β-catenin信号抑制肾癌细胞体外生长和侵袭的实验研究[J].海南医学院学报,2017,23(8):1034-1036,1040,4.基金项目
北京市科技计划课题(Z151100004015194) （Z151100004015194）
It was supported by Beijing Science and Technology Project (No.Z151100004015194). （No.Z151100004015194）
