核农学报2017，Vol.31Issue(7)：1245-1254,10.DOI:10.11869/j.issn.100-8551.2017.07.1245
玉米HSP70基因家族的全基因组鉴定与分析
Genome-wide Identification and Analysis of HSP70 Gene Family in Maize
摘要
Abstract
Heat shock protein 70 (HSP70) is a group of proteins induced by various environmental stresses,and exist in nearly all living organisms.To comprehensively analyze information for the ZmHSP7O genes of maize genome,HSP70 domain (PFO0012) downloaded from Pfam database was employed to identify ZmHSP70 genes in Gramene database.The obtained amino acid sequences were analyzed with the bioinformatics software or tools online.Totally 35 ZmHSP7O genes were found in maize genome and named from ZmHSP70-1 to ZmHSP70-35.These genes were distributed unequally in ten chromosomes of maize,the maximum of ZmHSP70 genes were found on chromosomes 5 (six genes) and only 1 gene was located on chromosomes 9.The result of gene structures and phylogenetic analysis revealed that 35 ZmHSP70 genes were classified into six group and the type,order,and number of motifs were similar in proteins with in the same group,but were differed from the proteins in other groups.The combined phylogenetic tree revealed that there were 8 pairs of orthologous genes and 17 pairs of paralogous genes among 86 HSP70 members in different organism.Among which 7 pairs of orthologous genes from maize and rice and 1 pair from rice and Arabidopsis.These results suggested that the orthologous pair originated from the common ancestral genes existed before the divergence of the monocots and dicots.Expression profile analysis indicated that most of ZmHSP70 genes were expressed constitutively,but expression patterns and expression quanlities were different.These results could provide a theoretical basis for further research on the function analysis of ZmHSP70 gene family.关键词
玉米/HSP70/生物信息态/系统进化树/表达分析Key words
maize/HSP70/bioinformatics/phylogenetic tree/expression analysis引用本文复制引用
宋晋辉,马海莲,瓮巧云,袁进成,赵艳,刘颖慧..玉米HSP70基因家族的全基因组鉴定与分析[J].核农学报,2017,31(7):1245-1254,10.基金项目
国家科技重大专项(2014ZX0800909B),河北省高等学校科技研究项目(QN2015246),河北省高等学科拔尖人才选拔与培养计划(BR2-234) （2014ZX0800909B）
