基础医学与临床2017，Vol.37Issue(6)：834-838,5.
Roux-en-Y胃旁路术对肥胖大鼠肾脏糖异生的影响
Effect of Roux-en-Y gastric bypass surgery on renal gluconeogenesis in obese rats
摘要
Abstract
Objective To investigate the effect of Roux-en-Y gastric bypass surgery on renal gluconeogenesis, and to explore the mechanism of improving glucose control and insulin sensitivity in obese rats.Methods Rats were randomly divided into the obese group (n=30) consumed high-fat diet (HFD) and control group (NC group, n=8).Top 24 of animals fed HFD by change in body mass were subdivided into three groups: obese control group (OB group, n=8), obese under RYGB group (RYGB group, n=8) or sham-operation (sham group, n=8).Intraperitoneal glucose tolerance tests (IPGTT) were performed meanwhile the AUC of blood glucose concentration-time after surgery.Fasting glucose, insulin and lipid were measured respectively and HOMA-IR were calculated.The mRNA level and protein level of the key enzymes (G6P and PEPCK) of renal gluconeogenesis were examined with RT-PCR and Western blot respectively.Results Compared with OB group and Sham group, the body mass, lipid, blood glucose, insulin, HOMA-IR and AUG were significantly decreased (P<0.05), the expression of G6P and PEPCK mRNA and protein were significantly reduced(P<0.05) in RYGBgroup.Conclusions Roux-en-Y gastric bypass surgery can improve glucose control and insulin resistance, which may be related to the decreased level of mRNA, protein of G6P and PEPCK, reduced gluconeogenesis and glucose output in renal cortex.关键词
胃旁路术/肥胖症/肾脏/糖异生Key words
Roux-en-Y gastric bypass/obesity/kidney/gluconeogenesis分类
医药卫生引用本文复制引用
郭伟,张书平,母松,汪志红,张军..Roux-en-Y胃旁路术对肥胖大鼠肾脏糖异生的影响[J].基础医学与临床,2017,37(6):834-838,5.基金项目
重庆市科技攻关应用技术研发项目(CSTC2012ggyyjs10038) （CSTC2012ggyyjs10038）
重庆市自然科学基金(CSTC2012jjA10040) （CSTC2012jjA10040）
