吉林农业大学学报2017，Vol.39Issue(2)：214-221,8.DOI:10.13327/j.jjlau.2016.3179
日本乙型脑炎病毒对干扰素诱导基因ISG15表达的影响
Effects of Japanese Encephalitis Virus on Expression of Interferon-stimulated Gene 15
摘要
Abstract
To investigate the interaction between Japanese encephalitis virus (JEV) and interferonstimulated genes,swine testicular (ST)cells were first treated with interferon α and infected with Japanese encephalitis virus.The results showed that mRNA levels of Mxl,Mx2,OAS1,OAS2 and interferon-stimulated gene 15 (ISG15) were significantly up-regulated except PKR,and the mRNA level of ISG15 was the highest among these.The results of viral genomes and titers showed that replication of JEV was significantly inhibited by overexpression of ISG15 compared with untreated ST cells,and viral yield was significantly increased by shRNA targeting the ISG15 gene.In the end,eukaryotic expression vector (pcDNA3.1) carrying capsid (C),membrane (M),envelope (E) or6 nonstructural genes (NS1,NS2A,NS2B,NS3,NS4A or NS4B) of JEV were constructed and transfected into ST cells to investigate effect on expression of ISG15.ISG15 mRNA level was significantly increased in ST cells tansfected with E and NS3 genes compared with other genes of JEV and the control.The results implied that a high level of ISG15 was correlated with the expression of JEV E and NS3 gene.In total,this study enables elucidation of the molecular mechanism of ISG15 against JEV,which will be useful for developing a novel treatment to combat JEV infection.关键词
日本乙型脑炎病毒/干扰素刺激基因/ISG15/E基因/NS3基因Key words
Japanese encephalitis virus/interferon-stimulated gene/ISG15/E gene/NS3 gene分类
生物科学引用本文复制引用
杨晶,华涛,唐波,唐应华,侯继波,张道华,黄克和..日本乙型脑炎病毒对干扰素诱导基因ISG15表达的影响[J].吉林农业大学学报,2017,39(2):214-221,8.基金项目
江苏省农业支撑计划项目(BE2012370),江苏省农业自主创新项目[CX(14)5044],公益性行业(农业)科研专项(201303046) （BE2012370）
