吉林农业大学学报2017，Vol.39Issue(3)：292-298,312,8.DOI:10.13327/j.jjlau.2016.3416
荔枝椿平腹小蜂触角味觉受体基因的鉴定与表达分析
Identification and Expression Analysis of Antennal Gustatory Receptor Genes of Anastatus japonicus
摘要
Abstract
The insect has extremely sharp taste perception system,and in insect feeding,mating and oviposition and other behavioral responses,it plays an important role.As an important parasitoid of Hymenoptera,Anastatus japonicus contributes to the prevention of Tessaratoma papillosa.Transcriptome sequencing of Anastatus japonicus antenna was conducted,and five gustatory receptor genes were obtained from the database by screening and identification.With RT-PCR,expressions of gustatory receptors of Anastatus japonicus female antennae,male antennae,thorax,abdomen and leg were compared.The results show that expressions of AjapGR58,AjapGR49 and AjapGR64f were identical and lower in different tissues of adults whereas expressions of AjapGR38 and AjapGR2 were relatively high.At the same time,AjapGR38 was expressed only in female antennae and thorax,AjapGR2 were expressed in female antennae,male antennae,thorax and abdomen,but was not expressed in legs.Judging by the phylogenetic tree,AjapGR58 and AjapGR49 were homologous and polymerized with DmelGR33a and DmelGR43a,DmelGR28 and AjapGR38 were homologous and polymerized with DmelGR66a,indicating that AjapGR58,AjapGR49 and AjapGR38 are in the bitter receptor family,and may also perform the function of bitter taste.AjapGR64f was homologous with DmeGR15 and DmelGR64f and polymerized with DmelGR64a,and at the same time,AjapGR2 and DmelGR64a,DmelGR64b,DmelGR64c,DmelGR64f and DmelGR5 formed a cluster together,indicating that AjapGR64f and AjapGR2 are in the sweet receptor family,and may also function as a function of sweet taste.关键词
荔枝椿平腹小蜂/味觉受体/功能表达Key words
Anastatus japonicus/gustatory receptor/functional expression分类
生物科学引用本文复制引用
李婧,张俊杰,王寅亮,任炳忠..荔枝椿平腹小蜂触角味觉受体基因的鉴定与表达分析[J].吉林农业大学学报,2017,39(3):292-298,312,8.基金项目
吉林省自然科学基金项目(20150101068JC) （20150101068JC）
