寄生虫与医学昆虫学报2017，Vol.24Issue(1)：41-47,7.DOI:10.3969/j.issn.1005-0507.2017.01.006
家蝇幼虫抗茵肽粗提物对人肝癌HepG2细胞凋亡、钙离子浓度及线粒体膜电位变化的影响
EFFECT OF MUSCA DOMESTICA ANTIMICROBIAL PEPTIDES ON CELL APOPTOSIS, MITOCHONDRIAL MEMBRANE POTENTIAL, AND INTRACELLULAR CALCIUM CONCENTRATION IN HEPG2 CELLS
摘要
Abstract
In this paper,we investigate the effect of antimicrobial peptides extracts on HepG2 cells from the perspective of tumor cell apoptosis and its mechanism.Hoechst 33258 staining were used to observe the cell nuclear morphology of HepG2 after treatment with antimicrobial peptide from Musca domestica larva.Flow cytometry were used to analysis the effect of antimicrobial peptide on cell apoptosis,mitochondrial membrane potential and intracel|ular calcium concentration of HepG2 cells.HepG2 cells treated with Musca domestica antimicrobial peptides had a typical morphological characteristic apoptosis,such as nuclear cohesion,chromatin chemotaxis and nuclear fragmentation.HepG2 cells apoptosis rate were increased with decreased mitochondria membrane potential and increased calciumion concentration under the pressure of antimicrobial peptides from domestic flies.关键词
家蝇幼虫/抗菌肽粗提物/肝癌HepG2细胞/凋亡/机制Key words
Musca domestica/Antimicrobial peptides/HepG2 cells/Apoptosis/Mechanism引用本文复制引用
邢佳欣,郭姗,赵瑞君,朱爱玉,刘娟娟..家蝇幼虫抗茵肽粗提物对人肝癌HepG2细胞凋亡、钙离子浓度及线粒体膜电位变化的影响[J].寄生虫与医学昆虫学报,2017,24(1):41-47,7.基金项目
太原市科学技术发展计划项目(081049) （081049）
