江苏大学学报（医学版）2017，Vol.27Issue(3)：185-189,5.DOI:10.13312/j.issn.1671-7783.y170031
高迁移率族蛋白B1调控放疗残存胰腺癌细胞的干性获得
Role of HMGB1 in the acquisition of stemness ofpost-radiation residual pancreatic carcinoma cell line SW1990 in vitro
摘要
Abstract
Objective:To investigate the stem cell transformation of the HMGB1 in the post-radiation residual pancreatic carcinoma cell line SW1990 in vitro.Methods:With various radiation doses(0,4,8,10,12 Gy), ELISA assay was used to access the content of HMGB1 in the supernatant of pancreatic carcinoma cell line SW1990.With another various radiation doses(0,4,6,8,10 Gy), the proportion of the CD133+ cells in the post-radiation residual SW1990 cells were accessed by flow-cytometry (FCM).The 8 Gy post-radiation residual SW1990 cells were divided into four groups, including PBS group, supernatant+EP(ethyl pyruvate, inhibitor of HMGB1)group, supernatant group, HMGB1(100 ng/mL) group, then the proportion of the CD133+ cells in each group were examined by flow-cytometry.At the same time, the expression levels of SOX2 and OCT4 proteins were detected by Western blotting.Results:ELISA assay showed that the concentration of the HMGB1 in the supernatant of the post-radiation residual pancreatic carcinoma cell line SW1990 was the highest at 10 Gy radiation dose[(217.3±34.97)ng/mL].FCM assay showed that the CD133+ cells ratio was the highest at the 8 Gy radiation dose in the varies doses[(22.63±0.74)%].Compared with PBS group, the CD133+ relative ratio of the supernatant+EP group,supernatant group and HMGB1(100 ng/mL) group were (2.2±0.11),(3.2±0.19) and (6.4±0.32), respectively(F=143.4,P<0.01).Meanwhile supernatant group and HMGB1 (100 ng/mL) group up-regulated notably the expression levels of SOX2 and OCT4 (P<0.01).Conclusion:HMGB1 is detected in the supernatant of the post-radiation residual pancreatic carcinoma cell line SW1990 in vitro, and HMGB1 can positively induce the acquisition of stemness of post-radiation residual SW1990 cells in vitro.关键词
放疗/高迁移率族蛋白B1/胰腺癌/CD133Key words
radiation/high mobility group box-1 protein/pancreatic carcinoma/CD133分类
医药卫生引用本文复制引用
宋廉,张雅宁,陈雪莲,朱丽,石卉,王鸣,朱海涛,张礼荣,王冬青..高迁移率族蛋白B1调控放疗残存胰腺癌细胞的干性获得[J].江苏大学学报（医学版）,2017,27(3):185-189,5.基金项目
国家自然科学基金资助项目(81502663) （81502663）
江苏省社会发展项目(BE2015668) （BE2015668）
镇江市社会发展项目(SH2015053) （SH2015053）
镇江市重点实验室项目(SS2013017) （SS2013017）
江苏省六大人才高峰项目(2015-WSN-005) （2015-WSN-005）
江苏大学学生科研立项项目(15A361) （15A361）
