江苏大学学报（医学版）2017，Vol.27Issue(3)：209-212,4.DOI:10.13312/j.issn.1671-7783.y170106
EB病毒编码的BARF1基因通过JNK/c-Jun信号通路调节鼻咽癌细胞bcl-2的表达
Epstein-Barr virus encoded BARF1 gene regulatesprotooncogene bcl-2 expression through JNK/c-Jun signalpathway in human nasopharyngeal carcinoma cells
摘要
Abstract
Objective:To investigate the effect of Epstein-Barr virus encoded BARF1 gene on the activation of JNK/c-Jun signal pathway and bcl-2 expression in human nasopharyngeal carcinoma cells.Methods:Western blotting was applied to analyze the activation of JNK/c-Jun pathway and the expression of BCL-2 in BARF1-stably-expressing human nasopharyngeal carcinoma cell line CNE1 (CNE1-BARF1) and the control of CNE1 cell transfected by pSG5 vector(CNE1-SG).After blocked by the JNK special inhibitor SP600125, the activation of JNK/c-Jun and the expression of BCL-2 were also analyzed in CNE1-BARF1 cells.Results:Compared with the control CNE1-SG, the level of c-Jun and BCL-2 protein expression, and phosphorolization of JNK and c-Jun protein were significantly increased in CNE1-BARF1(P<0.05).The expression of c-Jun and BCL-2 protein were decreased after treated by inhibitor SP600125(P<0.05), and the level of c-Jun protein phosphorylation also decreased, but did not reach statistical significance(P>0.05).Conclusion:BARF1 could upregulate protooncogene bcl-2 expression through activating JNK/c-Jun signal pathway in human nasopharyngeal carcinoma cells.关键词
EB病毒/BARF1/鼻咽癌/JNK/c-Jun/AP-1/bcl-2Key words
Epstein-Barr virus/BARF1/nasopharyngeal carcinoma/JNK/c-Jun/AP-1/bcl-2分类
医药卫生引用本文复制引用
褚福营,张雪怡,张宇琼,徐美琴,陈巧林,成静,周天戟..EB病毒编码的BARF1基因通过JNK/c-Jun信号通路调节鼻咽癌细胞bcl-2的表达[J].江苏大学学报（医学版）,2017,27(3):209-212,4.基金项目
江苏大学高级技术人才科研启动基金资助项目(06JDG011) （06JDG011）
