扬州大学学报（农业与生命科学版）2017，Vol.38Issue(1)：1-5,5.DOI:10.16872/j.cnki.1671-4652.2017.01.001
基于马立克病毒的传染性喉气管炎病毒载体疫苗研制
Vectored infectious laryngotracheitis vaccine based on recombinant Marek's disease viruses
摘要
Abstract
In this study,a recombinant type Ⅰ Marek's disease virus vaccine strain (CVI988/Rispens) expressing EGFP reporter gene,rMDV-EGFP,was used as parent virus to develop recombinant MDV expressing infectious laryngotracheitis virus (ILTV) gB and gD gene.Generally,the expression box of EGFP reporter gene was replaced with ILTV gB and gD gene through homologous recombination method.The generated recombinant viruses were designated as rMDV-ILTgB and rMDV-ILTgD.The recombinant viruses were identified by PCR and indirect immunofluorescence test,and the result showed that the target genes had been functionally integrated into the MDV genome as expected.In the protection efficacy test,the results showed that rMDV-ILTgB and rMDV-ILTgD immunization could provide better pro tection for virulent ILTV challenge than the live-attenuated ILTV vaccine.The data,including morbidity,mortality and weight gain after challenge,showed significant differences between groups immunized with recombinant MDVs and nonvaccinated challenge group.Futhermore,significant differences also existed between groups immunized with recombiuant MDVs and live-attenuated ILTV vaccine when the data of mortality and weight gain were compared.关键词
鸡传染性喉气管炎病毒/重组马立克病毒/gB基因/gD基因/免疫效力Key words
infectious laryngotracheitis virus/recombinant Marek's disease virus/gB gene/gD gene/protective efficacy分类
农业科技引用本文复制引用
张小荣,刘怡,黄海琼,蒙杰,蒋叶林,吴艳涛..基于马立克病毒的传染性喉气管炎病毒载体疫苗研制[J].扬州大学学报（农业与生命科学版）,2017,38(1):1-5,5.基金项目
江苏省科技支撑计划项目(BE2014354) （BE2014354）
现代农业产业技术体系建设专项(CARS-41-K08) （CARS-41-K08）
江苏省高校优势学科建设工程项目(2014-05) （2014-05）
