扬州大学学报（农业与生命科学版）2017，Vol.38Issue(1)：12-15,34,5.DOI:10.16872/j.cnki.1671-4652.2017.01.003
大肠埃希菌F4菌毛凝集性单抗制备及抗原表位差异
Escherichia coli ETEC F4 pilus agglutinative single preparation and the difference between antigen epitope
摘要
Abstract
By adopting monoclonal antibody preparation,we obtained four hybridoma cells,named 8EH2,8EH3,9E10,11D4 respectively,which secret specific monoclonal antibody against Enterotoxigenic Escherichia coli (ETEC) F4 fimbriae steadily.Both immunoblotting and agglutination assays demonstrated that all four monoclonal antibodies reacted specifically to the purified F4 fimbriae and F4 Escherichia coli of different serotypes F4ab,F4ac and F4ad with the char acteristic of agglutinating the bacteria.While no across reaction with F18,F6(987P) and F5(K99) Escherichia coli Salmonella enteritidis 50 336 strains and their isolates was shown.They all belong to IgG1 antibody and can recognize the epitope of F4ac,among them 8EH2,8EH3,9El0 also can better recognize the epitope of F4ab.关键词
产肠毒素大肠埃希菌F4/凝集性/单克隆抗体/抗原表位Key words
Escherichia coli F4/agglutination activity/monoclonal antibody/epitope分类
医药卫生引用本文复制引用
马琰,王一亭,赵静,权国梅,宗秋芳,包文斌,夏芃芃,朱国强..大肠埃希菌F4菌毛凝集性单抗制备及抗原表位差异[J].扬州大学学报（农业与生命科学版）,2017,38(1):12-15,34,5.基金项目
科技部畜禽重大疫病防控与高效安全养殖综合技术研发重点专项(2016YFD0500900) （2016YFD0500900）
国家自然科学基金资助项目(31672579、30571374、30771603、31072136、31270171) （31672579、30571374、30771603、31072136、31270171）
江苏高校优势学科建设工程项目(2014-09) （2014-09）
中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室开放基金项目(SKLVBF201706) （SKLVBF201706）
