扬州大学学报（农业与生命科学版）2017，Vol.38Issue(1)：16-19,4.DOI:10.16872/j.cnki.1671-4652.2017.01.004
猪STC-1 siRNA设计及其基因沉默效率检测
Design and efficiency of gene silencing detection on pig STC-1 siRNA
摘要
Abstract
Three pairs of STC-1 siRNA and a pair of negative control siRNA were designed based on STC-1 mRNA sequence.After transfection of PK15 cell,efficiency of transfection and gene expression was observed by fluorescent tech nique,and QRT-PCR and western blot were used to determine siRNA in cells that gene silencing on STC-1 siRNA and protein expression level.The results showed that three siRNA on STC-1 had a higher fluorescence signal after the transfection cells;STC-1 mRNA and STC-1 protein expression levels were significantly lower in STC-132 cells than those in other groups (P＜0.01).The study suggested that the successfully designed STC-1 siRNA sequences can provide a foundation for subsequent control on STC-1 expression in the function of muscle development.关键词
猪/斯钙素/siRNA/基因沉默Key words
swine/stanniocalcina/small interfering RNA/gene silencing分类
农业科技引用本文复制引用
杨开典,陈偲,齐传翔,鞠辉明..猪STC-1 siRNA设计及其基因沉默效率检测[J].扬州大学学报（农业与生命科学版）,2017,38(1):16-19,4.基金项目
国家自然科学基金资助项目(31272405) （31272405）
江苏高校优势学科建设工程项目(2014-09) （2014-09）
江苏高校品牌专业建设工程项目(TAAP 2015-2018) （TAAP 2015-2018）
