扬州大学学报（农业与生命科学版）2017，Vol.38Issue(1)：30-34,5.DOI:10.16872/j.cnki.1671-4652.2017.01.007
MiR-133a-3p抑制人胃癌细胞的增殖、迁移及侵袭研究
Inhibitory effect of MiR-133a-3p on proliferation, migration and invasion in human gastric cancer cells
摘要
Abstract
The expression of microRNA-133a-3p in normal human gastric cell GES1 and six gastric cancer cell lines with different malignant potential were examined by real-time PCR.SGC-7901 was transfected with miR-133a-3p stably.Cell proliferation was measured by MTT assays,the migration was detected by wound assays,and the invasion was compared by transwell assays.Results showed that miR 133a-3p expression was shown to be greatly downregulated in human gastric cancer cells compared to normal gastric cells (P＜0.05),but there was no significant association with the degree of differentiation (P＞0.05).After transfection with miR-133a-3p,the cell proliferation,migration and invasion were significantly reduced (P＜0.05).In short,the development of gastric cancer is associated with miR-133a-3p,and miR-133a-3p can be used as a potential diagnostic marker for gastric cancer.关键词
胃癌/miR-133a-3p/增殖/迁移/侵袭Key words
gastric cancer/microRNA-133a-3p/proliferation/migration/invasion分类
医药卫生引用本文复制引用
邵娟,张蕾,王继军,卜平,孔桂美..MiR-133a-3p抑制人胃癌细胞的增殖、迁移及侵袭研究[J].扬州大学学报（农业与生命科学版）,2017,38(1):30-34,5.基金项目
国家自然科学基金资助项目(81673736) （81673736）
