扬州大学学报（农业与生命科学版）2017，Vol.38Issue(1)：45-51,7.DOI:10.16872/j.cnki.1671-4652.2017.01.010
4个线粒体基因在鲤所属国家种质鉴定中的应用及效果评价
Country property identification and its application assessment of common carp (Cyprinus carpio L) stocks though 4 mtDNA sequences
摘要
Abstract
In recent years,DNA barcoding has been widely used to species identification.However,whether it can be useful and how to identity the country property of Common carp (Cyprinus carpio L) has been rarely reported.Four mitochondrial genes sequences (CO Ⅰ,COⅡ,Cytb,and D-loop) were captured and country property of common carp was identified according to DNA barcoding method.The results showed that Cytb yielded higher barcoding gap compared to others,while COⅡ obtained the highest success rate under the best match approach.Based on four mtDNA sequences,the adjacent countries of phylogenetic trees were clustered into one clade.Considering the barcoding gap,false positive rate and false negative ratio,Cytb had more advantages for analyzing the common carp's country property compared to other 3 mtDNA sequences,although the identification was still not ideal.To sum up,decreasing the false negative rate and false positive rate for the common carp strain or location identification are the further work using DNA barcoding technology.关键词
鲤亚种/mtDNA序列/鲤所属国家/DNA条形码间隙Key words
common carp subspecies/mtDNA/common carp located country/DNA barcoding gap分类
生物科学引用本文复制引用
苏胜彦,董在杰,朱文彬..4个线粒体基因在鲤所属国家种质鉴定中的应用及效果评价[J].扬州大学学报（农业与生命科学版）,2017,38(1):45-51,7.基金项目
“十二五”国家科技支撑计划项目(2012BAD26B02) （2012BAD26B02）
现代农业产业技术体系建设专项(CARS-46-06) （CARS-46-06）
中国水产科学研究院基本科研业务费资助项目(2016RC-LX03) （2016RC-LX03）
