江西农业学报2017，Vol.29Issue(5)：1-4,4.DOI:10.19386/j.cnki.jxnyxb.2017.05.01
鱼腥藻PCC 7120中al3556蛋白的表达与纯化
Expression and Purification of Protein all3556 from Anabaena sp.PCC 7120
摘要
Abstract
Succinic semialdehyde dehydrogenase plays important physiological roles in the prokaryotes and eukaryotes.BLAST sequence analysis reveals that the gene all3556 in Anabaena sp.PCC 7120 encodes succinic semialdehyde dehydrogenase.The gene all3556 was cloned from the genome of Anabaena sp.PCC 7120 by conventional PCR.Then the plasmid of pET-28a-all3556 was constructed by ligating all3556 gene with pET-28a vector, and the expression of all3556 in the competent cells of Escherichia coli strain BL21 (DE3) was observed.The results of SDS-PAGE showed that the protein all3556 could high-efficiently express in soluble form in this system.The protein all3556 was purified by Ni-NTA affinity chromatography, its purity was more than 95%, and its yield was about 27 mg/g wet cell.This study will lay an important base for further illustrating the catalytic function and mechanism of all3556 protein in the future.关键词
鱼腥藻PCC7120/all3556蛋白/蛋白表达与纯化Key words
Anabaena sp.PCC 7120/all3556 protein/Protein expression and purification分类
生物科学引用本文复制引用
王小琴,李至敏,雷国风,李志敏..鱼腥藻PCC 7120中al3556蛋白的表达与纯化[J].江西农业学报,2017,29(5):1-4,4.基金项目
国家自然科学基金项目(31400054和31560250) （31400054和31560250）
江西省自然科学基金重大项目(20161ACB21012). （20161ACB21012）
