口腔颌面外科杂志2016，Vol.26Issue(5)：323-327,5.DOI:10.3969/j.issn.1005-4979.2016.05.004
miRNA-21在口腔鳞癌患者唾液中的表达及意义
Clinical Significance and Immunohistochemical Expression of miRNA-21
in Whole Saliva of OSCC Patients
顾小军 1王腾勇 2刘新庆 3徐速 1章根训1
作者信息
- 1. 新余市人民医院口腔科,江西 新余 338000
- 2. 新余市人民医院检验科,江西 新余 338000
- 3. 江西省人民医院口腔科,江西 南昌 330006
- 折叠
摘要
Abstract
Objective:To investigate the expression level of miRNA-21 in whole saliva from oral squamous cell carcinoma (OSCC) patients and their significance associated to tumor behavior. Methods: Thirty-eight male and twenty-six female OSCC patients aged from 29 to 76 years, were selected from January 2012 to June 2016. All OSCC patients were newly-diagnosed and treatment-free. Twenty-five healthy volunteers matching in sex and age were also enrolled as controls. MiRNA-21 expression in saliva was examined by Real-time PCR. Student t test was used to analyze the difference between OSCC group and control group. Results: The expressions of miRNA-21 in saliva was higher in OSCC patients (2.75 ±0.94) than those in the healthy control individual (0.78±0.45, P<0.01). Up-regulated miRNA-21 expression level was associated with the tumor cell differentiation (P<0.01) and lymph node metastasis (P<0.05). Receiver operating characteristic (ROC) curve of miRNA-21 in the saliva about cell differentiation resulted in a diagnostic sensitivity of 89.5% and specificity of 66.7%respectively. Conclusion: The expression level of miRNA-21 in whole saliva may be a valuable adjuvant parameter for predicting the growth and behavior of OSCC.关键词
口腔鳞癌/唾液/微小RNA/miRNA-21Key words
oral squamous cell carcinoma/saliva/microRNA/miRNA-21分类
医药卫生引用本文复制引用
顾小军,王腾勇,刘新庆,徐速,章根训..miRNA-21在口腔鳞癌患者唾液中的表达及意义[J].口腔颌面外科杂志,2016,26(5):323-327,5.
