棉花学报2017，Vol.29Issue(2)：147-156,10.DOI:10.11963/issn.1002-7807.201702004
棉花幼苗对低温胁迫的响应及抗冷机制初步研究
The Effect of Low Temperature Stress on the Growth of Upland Cotton Seedlings and a Preliminary Study of Cold-Resistance Mechanisms
摘要
Abstract
[Objective] Our research aimed to investigate the effects of low temperature on tissues of cotton and the physiological,biochemical and molecular mechanisms of response to low temperature.[Method] We measured biomass,antioxidant enzyme activity and soluble protein content of four cotton materials with significantly different chilling tolerances after 4 ℃ low temperature treatment.We also measured the relative expression of chilling resistance-related genes of different tissues of cotton cultivar Yu 2067 after 4 ℃ treatment.[Result] The low temperature treatment (4 ℃ for 24 h) resulted in greater growth inhibition of chilling-sensitive than chilling-tolerant materials,with inhibition of root growth higher than that of aerial parts.According to cell membrane permeability measurements,root cell membranes of the four tested cottons were relatively stable under low temperature stress.Compared with membranes of roots and stems,leaf cell membranes were more sensitive to lowtemperature stress.Leaf cell membranes of chilling-resistant cotton materials were more stable than those of chilling-sensitive ones.After low temperature stress,the cell membrane permeability of cotton leaves was negatively correlated with cold resistance,suggesting the use of this parameter as an indicator of cold resistance in cotton.SOD activity in the roots of chilling-resistant materials remained unchanged,whereas that of cold sensitive-ones decreased extremely significantly.POD and CAT activities decreased in the roots of two cotton materials after low temperature stress.The activities of two types of antioxidant enzymes declined less markedly in cold-resistant Yu 2067 than in cold-sensitive materials.The soluble protein content of leaves and stems of cold-resistant cotton remained basically unchanged but decreased in cold-sensitive materials.Expression analysis of five chilling tolerance-related genes in Yu 2067 revealed that more genes were up-regulated than down-regulated in leaves.The four genes up-regulated in leaves were 3FAD (fatty acid desaturase),stress-induced protein,putative R2R3-MYB transcription factor and bHLH1 transcription factor.In contrast,more genes were down-regulated than up-regulated in roots.In roots,the three down-regulated genes were bHLH1 transcription factor,stress-induced protein and expansin 2.These expression patterns were consistent with the observed root growth inhibition.[Conclusion] Our results imply that a relationship exists between up-regulation of the four cold resistance-related genes in leaves and inhibition of leaf growth under low temperature conditions.关键词
棉花/低温胁迫/生物量/细胞膜透性/抗氧化酶活性/抗冷相关基因表达Key words
cotton/low temperature stress/biomass/cell member permeability/antioxidase activity/chilling resistance-related gene expression分类
农业科技引用本文复制引用
王俊娟,喻树迅,王帅,陆许可,阴祖军,王德龙,樊伟莉,穆敏,郭丽雪,叶武威..棉花幼苗对低温胁迫的响应及抗冷机制初步研究[J].棉花学报,2017,29(2):147-156,10.基金项目
国家重点研发计划“七大农作物育种”(2016YFD0100203) （2016YFD0100203）
