南京农业大学学报2017，Vol.40Issue(3)：450-456,7.DOI:10.7685/jnau.201612021
云南天竺葵上发现番茄斑萎病毒
Tomato spotted wilt virus was found as a pathogen of geranium (Pelargonium hortorum)in Yunnan Province
摘要
Abstract
[Objectives] Some geraniums (Pelargonium hortorum)showing chlorotic and ring spots,which look like being infected by tospoviruses,were found in Kunming Botanical Garden,Yunnan Province.[Methods] The crude extracts of sampled geranium leaves were rub-inoculated to Nicotiana benthamiana plants and the systemic leaves of the inoculated plants were detected together with the original sample by Dot-blot ELISA after symptom observation.The symptomatic N.benthamiana leaves were further detected by RT-PCR.[Results] The systemic leaves of inoculated N.benthamiana plants showed obvious mosaic,leaf curling and necrosis after seven days post inoculation.The results of Dot-blot ELISA and RT-PCR detection indicated that they were infected by Tomato spotted wilt virus(TSWV).The RT-PCR products amplified by specific primers of TSWV N gene were sequenced and the sequence analysis revealed that it shared 99.74％ identity with TSWV-LE isolate at the nucleotide level.[Conclusions] The data above suggests that the sampled geraniums showing ring spots are infected by TSWV,which is the first report in China on the infection of TSWV in geraniums and useful for the virus control.关键词
天竺葵/番茄斑萎病毒/Dot-blot ELISA/RT-PCRKey words
geranium/Tomato spotted wilt virus (TSWV)/Dot-blot ELISA/RT-PCR分类
农业科技引用本文复制引用
朱敏,王泊婷,黄莹,李佳,陶小荣..云南天竺葵上发现番茄斑萎病毒[J].南京农业大学学报,2017,40(3):450-456,7.基金项目
国家自然科学基金项目(31501607) （31501607）
教育部高等学校博士点基金(20130097110004) （20130097110004）
中组部青年拔尖人才(2014TBB02) （2014TBB02）
