南京农业大学学报2017，Vol.40Issue(3)：514-520,7.DOI:10.7685/jnau.201610013
(-)-羟基柠檬酸调控鸡胚原代肝细胞脂滴沉积的机制
Mechanisms of (-)-hydroxycitric regulated lipid droplet deposition in primary chicken hepatocytes
摘要
Abstract
[Objectives] This study was aimed to investigate the effects of (-)-hydroxycitric [(-)-HCA] on the lipid droplet deposition and potential biochemistry mechanism in primary chicken embryo hepatocytes.[Methods] Primary chicken embryo hepatocytes were exposed to(-)-HCA treatment.Oil red staining was used to detect the count and area of lipid droplets in hepatocytes.In addition,the contents of triglyceride,glycogen and glucose in hepatocytes and the activities of glucose metabolism and respiratory chain related enzymes were determined.[Results] No significant difference was observed on the cell viability and cell death rate in primary chicken hepatocytes after (-)-HCA treated.Oil red O staining analysis showed (-)-HCA significantly decreased the number and total area of lipid droplets,meanwhile the triglyceride content significantly decreased in(-)-HCA treated group compared with that in the control group.(-)-HCA treatment significantly increased the glucose consumption in primary chicken hepatocytes,while the ATP-citrate lyase activity and acetyl coenzymy A (CoA) content in cytosol significantly decreased in (-)-HCA treated group.Compared with control group,(-)-HCA promoted the activities of glucokinase,phosphofructokinase-1,pyruvate kinase,pyruvate dehydrogenase(E1),citrate synthase,aconitase,succinate dehydrogenase,malate dehydrogenase,NADH dehydrogenase and ATP synthase remarkably.[Conclusions] (-)-HCA decreased lipid droplets accumulation by reducing the acetyl CoA supply,which was mainly achieved via inhibition of ATP-citrate lyase activity,and accelerating the energy metabolism in primary chicken hepatocytes.关键词
(-)-羟基柠檬酸/脂代谢/葡萄糖代谢/能量代谢/鸡胚原代肝细胞Key words
(-)-hydroxycitric/lipid metabolism/glucose metabolism/energy metabolism/primary chicken hepatocytes分类
农业科技引用本文复制引用
李龙龙,彭梦玲,王典,俞磊,葛重阳,马海田..(-)-羟基柠檬酸调控鸡胚原代肝细胞脂滴沉积的机制[J].南京农业大学学报,2017,40(3):514-520,7.基金项目
国家自然科学基金项目(31572483) （31572483）
江苏省自然科学基金项目(BK20151434) （BK20151434）
