内科理论与实践2017，Vol.12Issue(2)：135-139,5.DOI:10.16138/j.1673-6087.2017.02.014
血红蛋白在三氧化二砷抑制血小板聚集中的功能研究
Effect of hemoglobin on inhibitory effect of arsenic trioxide on platelet aggregation
摘要
Abstract
Objective To detect the combining of hemoglobin with arsenic and analyze the effect of hemoglobin on inhibitory effect of arsenic trioxide (ATO) on platelet aggregation.Methods The combining of hemoglobin with paminophenylarsine oxide (PAPAO) was detected with electrospray ionization mass spectrometry (ESI-MS).Whole blood or platelet rich plasma (PRP) was incubated with ATO (0,10 μmol/L).Different concentrations of hemoglobin (0,40,60,80,100,120 g/L) were added into PRP,and incubated with ATO (0,10 μ mol/L).Then the platelet aggregation induced by adenosine diphosphate (ADP) (2 μmol/L) or collagen (2 mg/L) was measured.Results β chain of hemoglobin could combine with one molecule of PAPAO via a dehydration reaction.ATO could inhibit platelet aggregation in both PRP and whole blood,the inhibitory effect was significantly higher in PRP.Hemoglobin (≥ 80 g/L) could concentration dependently reverse the inhibition of platelet aggregation induced by collagen(r=0.956,P=0.003).Similarly,hemoglobin (≥ 80 g/L) could concentration dependently reverse the inhibition of platelet aggregation induced by ADP (r=0.940,P=0.005).Conclusions Hemoglobin reduces the inhibitory effect of ATO on platelet aggregation via binding to arsenic in a concentration-dependent manner.Hemoglobin plays an important role in the process of ATO inhibition of platelet aggregation.关键词
三氧化二砷/血小板/聚集功能/血红蛋白/急性早幼粒细胞白血病Key words
Arsenic trioxide/Platelet/Aggregation/Hemoglobin/Acute promyelocytic leukemia分类
医药卫生引用本文复制引用
崔雯,聂瑞敏,王瑾,奚晓东,糜坚青..血红蛋白在三氧化二砷抑制血小板聚集中的功能研究[J].内科理论与实践,2017,12(2):135-139,5.基金项目
上海市慈善癌症研究中心慈善慢跑课题 （）
