农业机械学报2017，Vol.48Issue(5)：94-100,7.DOI:10.6041/j.issn.1000-1298.2017.05.011
基于纸质微流控芯片的农药检测系统
Detection System for Pesticides with Paper-based Microfluidic Chip
摘要
Abstract
In order to solve the problems relating to pesticides detection with the use of complex;instruments in terms of high cost,tedious operation and low degree of automation,a new method for;pesticides detection with paper-based microfluidic chip was provided.The paper-based microfluidic chip;was designed and developed with automatic sample injection,hybrid reaction and electrochemical;detection.Subsequently,the enzyme electrode of ring structure was prepared by chemical crosslinking;method using graphite carbon and Ag/AgCl materials and then characterized with electrochemistry cyclic;voltammetry (CV).The integrated enzyme electrode of the paper-based microfluidic detection system for;pesticides was built based on enzyme inhibition mechanism.Finally,the linear model between inhibition;rate and parathion pesticides concentration was set up and the performance of the enzyme electrode was;tested.The results showed that the prepared enzyme electrode was of good repeatability,linearity and;stability.Furthermore,a linear relationship was displayed between inhibition rate and parathion;pesticides,which was represented by the equation I =158.82 +21.11lgC with good linear range of 1.0 ×10-7 g/mL ～ 1.0 × 10-5g/mL.The determination coefficient obtained was 0.993 and the corresponding;limit of detection was 3.3 × 10-8 g/mL.The enzyme electrode integrated on the paper-based microfluidic;chip was robust with parathion pesticides which can not be interfered with other pesticides and substance;with standard addition recovery rate of 95.8％ ～115.0％.关键词
农药检测系统/酶抑制法/纸质微流控芯片/酶电极/电化学检测Key words
pesticides detection system/enzyme inhibition method/paper-based microfluidic chip/enzyme electrode/electrochemical detection分类
农业科技引用本文复制引用
毛罕平,左志强,施杰,杨宁,HUANG J S,严玉婷..基于纸质微流控芯片的农药检测系统[J].农业机械学报,2017,48(5):94-100,7.基金项目
“十二五”国家科技支撑计划项目(2014BAD08B03)、国家自然科学基金重点项目(61233006)、国家自然科学基金项目(31671584)、江苏省农业科技自主创新资金项目(CXC1571033-02)和江苏省高校优势学科建设工程项目(苏财教(2014)37号) （2014BAD08B03）
