农业机械学报2017，Vol.48Issue(5)：193-198,6.DOI:10.6041/j.issn.1000-1298.2017.05.024
金属有机骨架化合物PCN222对酶抑制法的增敏研究
Porous Metal-organic Frameworks: PCN 222 Sensitization to Ache Inhibition Method
摘要
Abstract
Food safety issues caused by pesticide residues become the spotlight of public concerns all over the world.Metal-organic frameworks:porous coordination network 222 (MOFs:PCN 222) were synthesized and investigated as a novel sensitizer for AChE-inhibited method.TCPP (Tetrakis (4-carboxyphenyl)porphyrin) was employed as a ligand and highly stable Zr6 clusters were chosen as nodes for the assembly of stable Zr-MOFs.With carefully selected starting materials,a 3D porous MOFs was successfully constructed.Some properties of the prepared PCN 222,including structure,morphology X-ray diffraction (XRD) and thermostability were studied systematically.Porous virgate structure (spindle hexahedron),large surface area and strong thermostability were obtained for the MOFs.Based on the PCN 222,a sensitive AChE-inhibited method was developed.As a proof of concept,chlorpyrifos was used as a model analyte.Under the optimal conditions,absorbance increasing efficiency was increased by 68.8％ (in 3 min),which substantially improved the sensitivity of the colorimetric method compared with traditional ache inhibition method.The results revealed that the sensitization range obtained for chlorpyrifosis was approximately 0.01 ～ 10 μg/mL.Furthermore,the biosensor was successfully applied to the quantitative assay of chlorpyrifos in synthetic sample (vegetable matrix).In addition,the method possessed the advantages of simplicity,rapidity and cost-effective.Hence,the developed sensitized AChE-inhibited method might provide a useful and practical tool for organophosphorus pesticide determination and related food safety analysis.关键词
金属有机骨架化合物/多孔配位网络结构222/毒死蜱/酶抑制法/增敏Key words
metal-organic frameworks/porous coordination network 222/chlorpyrifos/AChE-inhibited method/sensitization分类
农业科技引用本文复制引用
徐霞红,郭玉娜,王新全,王祥云,齐沛沛,汪志威..金属有机骨架化合物PCN222对酶抑制法的增敏研究[J].农业机械学报,2017,48(5):193-198,6.基金项目
国家自然科学基金项目(31671940) （31671940）
