农业机械学报2017，Vol.48Issue(5)：313-319,334,8.DOI:10.6041/j.issn.1000-1298.2017.05.039
枸杞枝条发酵木质纤维素降解与微生物群落多样性研究
Characteristics of Degradation of Lignocellulose and Microbial Community Diversity during Fermentation of Wolfberry Branches Substrate
摘要
Abstract
The Chinese wolfberry branch,normally as agricultural wastes,is one of the most important renewable and reuseable resources of plant fibers in Ningxia.To improve the efficiency of wolfberry branch utilization,the characteristics of lignocellulose degradation,microbial community metabolism and diversity were studied during the fermentation of wolfberry branches substrate.By design of orthogonal experiment,wolfberry powder branches and sophora alopecuroides stem powder mixed in ratio of 4∶1 were used to study the influence of fermentative factors on microbial community structure and diversity during the fermentation by Biolog-ECO system.The results showed that at the end of the fermentation,the degradation rates of cellulose,hemicellulose and lignin were stayed above 15％,19％ and 10％,respectively.The treatment,which added oil cake and inoculated with coarse cellulose degrading bacteria,got the higher degradation rates of lignocellulose at temperature of 60℃,moisture content of 60％,and the degradation rates of cellulose,hemicellulose and lignin were 18.12％ ～ 19.22％,23.55％ ～25.21％ and 13.87％ ～ 14.24％,respectively,which were increased significantly than those of the other treatments;microbial activity and diversity during high temperature period were increased.Average well color development of microbes were 1.019,1.062,0.943 and 1.117,and ShannonWiener index,Simpson index and richness index of microbes were 2.321 ～ 2.365,0.930 ～0.941 and 18.78 ～ 20.33,respectively.The ability to metabolize microorganism on part of carbon source was improved,which resulted in promoting degradation of organic matter.关键词
枸杞枝条粉/木质纤维素/降解/微生物/群落多样性/平均颜色变化率Key words
wolfberry branches powder/lignocellulose/degradation/microbes/community diversity/average well color development分类
化学化工引用本文复制引用
冯海萍,杨冬艳,白生虎,谢华,刘晓梅,裴红霞..枸杞枝条发酵木质纤维素降解与微生物群落多样性研究[J].农业机械学报,2017,48(5):313-319,334,8.基金项目
国家自然科学基金项目(31501803)和宁夏农林科学院科技创新先导资金项目(NKYQ-16-05) （31501803）
