农业机械学报2017，Vol.48Issue(5)：350-355,6.DOI:10.6041/j.issn.1000-1298.2017.05.044
乐果农药对生菜叶片微观结构的作用机理研究
Mechanism of Different Concentrations of Dimethoate Residue on Microstructure of Lettuce Leaves
摘要
Abstract
Fast identification of pesticide residue in lettuce leaves plays a key role in the test of food safety.However,there are a few studies on the mechanism of pesticide on the internal structure of lettuce.In order to further study the effects of dimethoate on lettuce microstructure,the lettuce at rosette stage was used as the experimental object.Besides,scanning electron microscopy (SEM) and transmission electron microscopy (TEM) were used to detect the microstructure of lettuce leaves.It was found that the microstructure of the lettuce leaves was changed obviously with the increase of the concentration of dimethoate pesticides sprayed.Moreover,the ratio of length to width and density of stomata in leaves were decreased,leaf thickness was decreased,osmiophilic granules were increased and the starch granules were decreased.In the wavelength range of 897 ～ 1 332 nm,spraying dimethoate lettuce spectral reflectance was inversely proportional to the concentration of dimethoate.However,in the wavelength range of 1 703 ～ 1 754 nm,spraying dimethoate lettuce reflectance was proportional to the concentration of dimethoate.In addition,dimethoate pesticide spraying with different concentrations resulted in different textures of lettuce leaves.The results showed that dimethoate produced a huge change on lettuce microstructure,which caused great changes of the image information of lettuce leaves.Study on the microstructure of lettuce leaves sprayed with different concentrations of dimethoate pesticide provided the basis for the study on the mechanism of HSI technology to detect the different concentrations of pesticide residues in lettuce.Therefore,hyperspectral image detection technology can effectively realize the quantitative detection of pesticide residues on the surface of lettuce leaves.关键词
生菜叶片/农药残留/机理/微观结构/特征提取/高光谱图像Key words
lettuce leaves/pesticide residues/mechanism/microstructure/feature extraction/hyperspectral image分类
农业科技引用本文复制引用
孙俊,周鑫,李青林,蒋淑英,毛罕平,杨宁..乐果农药对生菜叶片微观结构的作用机理研究[J].农业机械学报,2017,48(5):350-355,6.基金项目
国家自然科学基金项目(31471413)、江苏高校优势学科建设工程项目PAPD(苏政办发2011 6号)、江苏大学现代农业装备与技术重点实验室开放基金项目(NZ201306)和江苏省六大人才高峰项目(ZBZZ-019) （31471413）
