农药学学报2017，Vol.19Issue(3)：298-306,9.DOI:10.16801/j.issn.1008-7303.2017.0039
海南省芒果可可球二孢对多菌灵的敏感性及菌株适合度研究
Sensitivities of carbendazim-resistance and fitness of Botryodiplodia theobromae strains from mango in Hainan
摘要
Abstract
Strains of Botryodiplodia theobromae collected from mango in Hainan of China were used to characterize their resistance to carbendazim and fitness. The resistance of 90B. theobromae strains to carbendazim was detected using distinguishing dosage method. Fitness of resistant strains was compared with sensitive strains from the aspects of mycelial growth rate, pathogenicity, osmotic pressure and electroosmosis rate. The results showed that the resistance frequency of strains to carbendazim was 65.56% and the major resistant type is of high resistance (8 medium resistant strains and 51 high resistant strains). TheB. theobromae strains have high hazard to mango. And there were 65 highly virulent strains accounted for 72.22% of the whole population. The mycelial growth rates of the resistant strains and the sensitive strains were significantly different at the 0.01 level between. However, there were not significant differences in the pathogenicity. The relative conductivity of sensitive strains mycelia was higher than that of the resistant strains. It indicated that the cell membrane permeability of the sensitive strains was higher, which led to the leakage of a large number of cell electrolyte .This study showed that strain of B. theobromae has high resistance to carbendazim, the fitness of resistance strains is better than that of sensitive strains.关键词
芒果蒂腐病/可可球二孢/多菌灵/抗药性/适合度Key words
mango stem-end rot/Botryodiplodia theobromae/carbendazim/fungicide resistance/fitness分类
农业科技引用本文复制引用
赵磊,杨叶,王萌,贺瑞,陈绵才..海南省芒果可可球二孢对多菌灵的敏感性及菌株适合度研究[J].农药学学报,2017,19(3):298-306,9.基金项目
国家自然科学基金(31560521) （31560521）
海南省属科研院所技术开发研究专项(KYYS-2015-01). （KYYS-2015-01）
