农药学学报2017，Vol.19Issue(3)：316-323,8.DOI:10.16801/j.issn.1008-7303.2017.0041
高效氯氰菊酯亚致死浓度对桃小食心虫成虫体内解毒酶活性的影响
Effect of sublethal concentration of beta-cypermethrin on activities of detoxifying enzymes inCarposina sasakii Matsumura (Lepidoptera: Carposinidae) adults
摘要
Abstract
To investigate the effects of sublethal concentration ofbeta-cypermethrin on activities of detoxifying enzymes inCarposina sasakii Matsumura adults, theC. sasakii adults were treated with LC10, LC20 or LC40 concentrations ofbeta-cypermethrin using residual film method. The activities of three detoxifying enzymes were then determined. 24 h after the treatments, the adults were transferred to clean conditions. The activities of the carboxylesterase (CarE), glutathioneS-transferases (GSTs) and ethoxycoumarinO-de-ethylase (ECOD) in the adults mentioned above were investigated after 0, 6, 12 and 24 h, respectively. The results showed that activities of ECOD were inhibited significantly 24 h after the treatments. As thebeta-cypermethrin concentration increases, the activities of ECOD were induced gradually especially for the LC40-beta-cypermethrin treated adults whose ECOD activity was significantly higher than that of thebeta-cypermethrin-free controls. Additionally, the activities of CarE and GSTs in male moths increased. The activities were inhibited in females after treated with LC20 or LC40 concentration ofbeta-cypermethrin. Afterbeta-cypermethrin treatment, the activities of the detoxifying enzymes were increased at the beginning and then decreased. Moreover, the activities of three detoxifying enzymes in adults fluctuated more significantly with the increase ofbeta-cypermethrin concentrations and distinct sex differences may exist. According to the short-term physiological responses of theC. sasakii adults tobeta-cypermethrin, the above mentioned three detoxifying enzymes in female or male moths play a comparatively active role for it and the activities of the detoxifying enzymes were increased at first and then decreased. Different physiological function of female and male moths, as well as the differences in susceptibility to insecticides, resulted in the dosage and sex differences of theC. sasakii adults responses to the sublethal concentrations of thebeta-cypermethrin.关键词
桃小食心虫/高效氯氰菊酯/亚致死浓度/解毒酶Key words
Carposina sasakii/beta-cypermethrin/sublethal concentration/detoxifying enzymes分类
农业科技引用本文复制引用
全林发,仇贵生,孙丽娜,李艳艳,闫文涛,岳强,张怀江..高效氯氰菊酯亚致死浓度对桃小食心虫成虫体内解毒酶活性的影响[J].农药学学报,2017,19(3):316-323,8.基金项目
国家重点研发计划"苹果化肥农药减施增效技术集成研究与示范"(2016YFD0201100) （2016YFD0201100）
果树产业技术体系辽宁创新团队(LNGSCYTX). （LNGSCYTX）
