器官移植2017，Vol.8Issue(3)：200-204,5.DOI:10.3969/j.issn.1674-7445.2017.03.005
雄性大鼠肝移植术后生育模型的建立
Establishment of male rat models for fertility after liver transplantation
摘要
Abstract
Objective To establish male rat models for fertility following liver transplantation. Methods Male Sprague-Dawley (SD) rats were used as the donors and recipients of liver transplantation. The donor liver was transplanted with two-cuff technique. Liver transplantation was performed in 15 male SD rats. At 3 weeks after liver transplantation, 5 rats were randomly sacrificed for detection of sperm deformity rate. The remaining male rats were mixed bred and mated with healthy female SD rats at a ratio of 1︰2. General conditions of the rats undergoing liver transplantation were recorded. Liver function parameters were detected after liver transplantation. Postoperative sperm deformity rate was observed. The pregnant status of female rats and health situation of their offsprings was monitored. Results All 15 rats (100%) underwent liver transplantation successfully. Nine rats (9/10) survived longer than 8 weeks. Liver function parameters were normal in male rats following liver transplantation. The sperm deformity rate was ranged from 0.5% to 1.3%. Ten male rats undergoing liver transplantation were mixed bred with female rats at a ratio of 1︰2 for 1 week. All female rats were successfully mated and delivered their offsprings after 3 weeks. The offsprings had no evident physiological deformity. Conclusions Male rat models for fertility are successfully established after liver transplantation, which serve as an animal model to evaluate the fertility performance in male patients undergoing liver transplantation.关键词
肝移植/生育模型/SD大鼠/雄性/疾病模型/动物模型/器官移植/显微外科手术Key words
Liver transplantation/ Fertility model/ SD rat/ Male/ Disease model/ Animal model/ Organ transplantation/ Microscopic surgery分类
医药卫生引用本文复制引用
谌小龙,汪根树,张俊斌,林国莉,冯智英,金海,杨建旭..雄性大鼠肝移植术后生育模型的建立[J].器官移植,2017,8(3):200-204,5.基金项目
广东省科技计划项目 (2011B060300002) （2011B060300002）
