器官移植2017，Vol.8Issue(3)：205-208,4.DOI:10.3969/j.issn.1674-7445.2017.03.006
不同品系小鼠间皮肤移植致敏后抗供体特异性抗体的变化特点研究
Study on variation characteristics of anti-donor specific antibodies in different species of sensitized mice after skin transplantation
摘要
Abstract
Objective To compare the change features of anti-donor specific antibody (DSA) in different species of sentitized mice after skin transplantation. Methods All mice were divided into the Balb/c → C57BL/6 (6 pairs) and Balb/c → C3H skin transplantation groups (6 pairs). At d0, d2, d4, d7, d13, d17, d28, d35, d42, d49 and d56 after skin transplantation, the serum sample was prepared for detection of DSA-IgG and DSA-IgM levels. Results Moderate increase was noted in the DSA-IgG level in the sensitized mice within 1 week after skin transplantation. The IgG level was significantly increased within 1-4 week and peaked and stabilized within 4-8 week. No significant variation was observed in the DSA-IgM level at 8 weeks after skin transplantation. In the Balb/c → C57BL/6 skin transplantation group, the DSAIgG level was significantly lower than that in the Balb/c → C3H group. Statistical significance was identified in the IgG levels between two groups at d2, d17, d28, d35, d42, d49 and d56 after skin transplantation (all P<0.05). No statistical significance was noted in the DSA-IgM levels between two groups at each time point (all P>0.05). Conclusions Advancing the time of renal transplantation after skin transplantation moderately in the Balb/c → C3H group, or changing to the lower immunoreactive combination of Balb/c → C57BL/6 are aimed to establish AMR mouse models with mild rejection reaction.关键词
皮肤移植/供体特异性抗体(DSA)/抗体介导的排斥反应(AMR)/小鼠/免疫反应/肾移植/动物模型/免疫球蛋白(Ig)Key words
Skin transplantation/Donor-specific antibody (DSA)/Antibody-mediated rejection (AMR)/Mouse/Immune response/Renal transplantation/Animal model/Immunoglobulin (Ig)分类
医药卫生引用本文复制引用
廖涛,赵大强,李思雯,韩飞,罗子寰,刘潇楠,孙启全..不同品系小鼠间皮肤移植致敏后抗供体特异性抗体的变化特点研究[J].器官移植,2017,8(3):205-208,4.基金项目
教育部博士点基金新教师类(20130171120100) （20130171120100）
国家自然科学基金面上项目(81470978) （81470978）
广东省自然科学基金重点项目(2015A030311040) （2015A030311040）
