热带作物学报2017，Vol.38Issue(3)：403-407,5.DOI:10.3969/j.issn.1000-2561.2017.03.003
濒危华石斛种子活力测定方法研究
Determination of Seed Viability in Endangered Orchid of Dendrobium sinense
摘要
Abstract
Dendrobium sinense is an endangered orchid species,which has an important ornamental and medicinal value.The seed is tiny and with an undifferentiated embryo,which making it difficult to store and loss its vitality rapidly.Therefore screening of a fast,accurate methods for detecting seed viability is an important prerequisite to explore the effective preservation method of orchid seeds.An experiment using 2,3,5-triphenyltetrazolium chloride (TTC) and ultraviolet spectrophotometer (UVS) was studied through determined the staining rate and absorbance.The results show that:In the preservation of 1 d to 30 d,the seed viability determined using TTC had an average germination rate of 0.6％ higher than the control,with a change range less than 0.4％,but the operation is complicated.However,the seed viability using UVS had an average germination rate of 0.04％ higher than the control,with a large change range as high as 3.09％,but the experimental operation is simple.Conclusion:The method of TTC could accurately measure the seed viability of D.sinense,but time-consuming,and the method of UVS is simple and rapid,while the accuracy was slightly lower than that of TTC.关键词
华石斛/种子活力/四氮唑法(TTC)/紫外分光光度计法(UVS)/吸光度Key words
Dendrobium sinense/seed viability/triphenyltetrazolium chloride (TTC)/ultraviolet spectrophotometer (UVS)/absorbance分类
医药卫生引用本文复制引用
边子星,颜彩燕,姚肖健,杨福孙..濒危华石斛种子活力测定方法研究[J].热带作物学报,2017,38(3):403-407,5.基金项目
国家自然科学基金“濒危附生兰华石斛原地共生萌发及成苗机理研究”(No.31160399) （No.31160399）
国家自然科学基金“两种生境下濒危植物五唇兰的无性与有性繁殖对策及幼苗更新能力差异”(No.31660227) （No.31660227）
海南省普通高校研究生创新科研课题“华石斛原球茎共生成苗营养生理机理研究”(No.Hys2015-05). （No.Hys2015-05）
