热带作物学报2017，Vol.38Issue(3)：508-512,5.DOI:10.3969/j.issn.1000-2561.2017.03.020
大根唇柱苣苔的组培快繁技术
Tissue Culture and Rapid Regeneration of Chirita macrorhiza
摘要
Abstract
The leaf of Chirita macrorhiza was used as the explants and cultured on MS medium supplemented with different concentrations of plant hormones.The results showed that:the adventitious bud induction culture medium was MS+6-BA 1.00 mg/L+NAA 0.05 mg/L.The shoots proliferation medium was MS+6-BA 1.50-3.00 mg/L+NAA 0.10 mg/L.The root induction medium was 1/2MS+IBA 0.20-0.30 mg/L.The suitable ratio of transplanting medium for peat ∶ perlite was 2 ∶ 1,peat ∶ fine sand is 2 ∶ 1,and peat ∶ soil was 2 ∶ 1.关键词
大根唇柱苣苔/组培/快繁/不定芽Key words
Chirita macrorhiza/tissue culture/rapid regeneration/adventitious bud分类
农业科技引用本文复制引用
林茂,李进华,唐庆,王华新,廖美兰,龙定建..大根唇柱苣苔的组培快繁技术[J].热带作物学报,2017,38(3):508-512,5.基金项目
广西林业科技项目(桂林科字2013第5号) （桂林科字2013第5号）
广西自然科学基金项目(No.2014GXNSFBA118091) （No.2014GXNSFBA118091）
国家林业局部门预算广西项目(桂林护预2015007) （桂林护预2015007）
广西壮族自治区林业科学研究院基本科研业务费项目(林科201405号). （林科201405号）
