热带作物学报2017，Vol.38Issue(3)：513-520,8.DOI:10.3969/j.issn.1000-2561.2017.03.021
基于RNAi抗PRSV番木瓜株系的培育及其分子特征验证
Breeding and Molecular Characteristion of RNAi Papaya for Resistance of Papaya Ringspot Virus
摘要
Abstract
RNAi-mediated virus resistance strategy was used to develop PRSV resistance papaya lines.A plant expression vector pCAMBIA2300-35S-CP-RNAi-OCS was constructed.The vector was transferred into papaya callus by particle bombardment,selected on kanamycin media.Transformation papaya lines were validated by PCR,RT-PCR,ddPCR,etc.The transgenic lines were further challenged with viral inoculation.The results showed that transgenic line 474 was single copy insertion and insertion site was located in papaya chromosome 7 supercontig_61 at 717 141 bp;non-transgenic line 1280 accumulated higher concentration of virus and developed symptoms,while transgenic line 474 was almost no virus accumulation.Transgenic line 474 was resistance to PRSV,which needed field test.关键词
番木瓜环斑病毒/番木瓜/RNAiKey words
Papaya ringspot virus (PRSV)/papaya/RNAi分类
农业科技引用本文复制引用
黄静,贾瑞宗,孔华,郭静远,王绪朋,郭安平..基于RNAi抗PRSV番木瓜株系的培育及其分子特征验证[J].热带作物学报,2017,38(3):513-520,8.基金项目
农业部948项目(No.2014-Z17 （）
No.2015-Z23) （）
海南省重点项目(No.ZDYF2016027) （No.ZDYF2016027）
海南省自然科学基金(No.20153145). （No.20153145）
