热带作物学报2017，Vol.38Issue(3)：521-528,8.DOI:10.3969/j.issn.1000-2561.2017.03.022
山蚂蝗属植物遗传多样性的AFLP分析
Genetic Diversity of Desmodium Desv.on AFLP Analysis
摘要
Abstract
In order to classify the genetic diversity and genetic relationship of Desmodium species,46 Desmodium Desv.lines were studied using amplified fragment length polymorphism (AFLP) markers.Two primer combinations (E-AAC/M-CAA,E-AAG/M-CAA) with higher polymorphism were selected from 64 combinations.A total of 172 AFLP markers were generated from the selected two primer combinations and of which,154 were polymorphic (89.53％),indicating that the genetic diversity of Desmodium was abundant.According to AFLP marker data,the experimented 46 materials were clustered into 6 groups by using NTSYS software,and the cluster analysis results were consistent with the classification system published in-the Chinese flora.D.ovalifolium and D.strgillosum may also be classified to the Subgen.Sagotia (Duchass.et Walpers) Baker.D.ovalifolium and D.strgillosum was closely related,and similar results were observed between D.strgillosum and D.heterocarpon.In contrast,relationships between the introduced species-CIAT350 (29) and other D.ovalifolium materials were far.Overall,there was not much relationship between the clustering results and the geographical origin.Regarded to AFLP technique,it is noted in efficiency and replicate,stable results as well as more information,which contributes to the genetic diversity analysis and identification of Desmodium species.关键词
山蚂蝗属/AFLP/遗传多样性/聚类分析Key words
Desmodium Desv/AFLP/genetic diversity/clustering analysis分类
农业科技引用本文复制引用
王春梅,任健,兰平秀,马向丽,李阳春,白昌军,徐立..山蚂蝗属植物遗传多样性的AFLP分析[J].热带作物学报,2017,38(3):521-528,8.基金项目
国家自然科学基金(No.31601997) （No.31601997）
国家自然科学基金项目子课题(No.K1400260). （No.K1400260）
