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木薯细菌性萎蔫病菌抗铜性评价及抗铜相关基因簇分子分析

时涛 蔡吉苗 李超萍 黄洁 黄贵修

热带作物学报2017,Vol.38Issue(3):529-540,12.
热带作物学报2017,Vol.38Issue(3):529-540,12.DOI:10.3969/j.issn.1000-2561.2017.03.023

木薯细菌性萎蔫病菌抗铜性评价及抗铜相关基因簇分子分析

Sensitivity Evaluation to Copper and Molecule Analysis of Copper Resistance Related Gene Clusters for Xanthomonas axonopodis pv.Manihotis

时涛 1蔡吉苗 1李超萍 1黄洁 2黄贵修1

作者信息

  • 1. 中国热带农业科学院环境与植物保护研究所 农业部热带作物有害生物综合治理重点实验室 海南省热带农业有害生物监测与控制重点实验室 海南海口571101
  • 2. 中国热带农业科学院热带作物品种资源研究所,海南儋州 571737
  • 折叠

摘要

Abstract

Cassava bacterial blight caused by Xanthomonas axonopodis pv.manihotis is one kind of worldly important diseases,and it is the most serious disease in the cassava plantations of China too.Further research results showed the copper-based fungicides had a little effective on cassava bacterial blight,so the resistance evaluation to copper and molecule analysis about copper resistance related gene clusters for X.axonopodis pv.manihotis was launched in our lab.The evaluation results showed the lowest effective concentration of 42 strains came from different areas were from 1.35 mmol/L to 1.75 mmol/L.Compared with reported copper resistance genes from other Xanthomonas spp strains,the two homologous gene clusters of copTAB and XmeRSA were anchored in the genome of X.axonopodis pv.manihotis strain GX11 from Guangxi province.These two gene clusters were also located in almost all of 67 strains from South America,Africa and Asia,and phylogenetic analysis showed this two gene clusters were highly consistent between strains.The results in this paper would facilitate for further research related with response mechanism of X.axonopodis pv.manihotis strain to copper and formulate the control program for this disease.

关键词

木薯/细菌性萎蔫病菌/copTAB基因簇/XmeRSA基因簇

Key words

cassava/Xanthomonas axonopodis pv.manihotis/copTAB gene clusters/XmeRSA gene clusters

分类

农业科技

引用本文复制引用

时涛,蔡吉苗,李超萍,黄洁,黄贵修..木薯细菌性萎蔫病菌抗铜性评价及抗铜相关基因簇分子分析[J].热带作物学报,2017,38(3):529-540,12.

基金项目

农业部现代农业人才支撑计划项目(No.0316001) (No.0316001)

国家现代木薯产业技术体系病害防控岗(No.CARS-12-hnhgx)和栽培管理岗(No.CARS-12-hnhj) (No.CARS-12-hnhgx)

中央级公益性科研院所基本科研业务费专项中国热带农业科学院院级创新团队项目(No.17CXTD-26)和院本级科研业务费专项(No.1630042016003). (No.17CXTD-26)

热带作物学报

OA北大核心CSCDCSTPCD

1000-2561

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