热带作物学报2017，Vol.38Issue(4)：646-651,6.DOI:10.3969/j.issn.1000-2561.2017.04.010
高温胁迫对华石斛幼苗的形态和生理参数的影响
Effects of High Temperature Stress on the Morphological and Physiological Parameters of Dendrobium sinense Seedlings
摘要
Abstract
High temperature is a major bottleneck in the cultivation of Dendrobium sinenese habiting the tropical cloud forest or montane rainforest with altitudes above 1 000 m in Hainan Island,China.To investigate how D.sinense responses to heat stress,morphological and physiological indices were measured for the seedlings grown under four temperatures,i.e.,25/20 (control),30/25,35/30 and 40/35 ℃ and a day/night cycles of 10h/14h for 1,2,4,8d,and recovery for 15d.Our results showed that no significant differences in morphological,physiological and biochemical changes were found in the seedlings under 30 ℃ and 35 ℃ for 1 d,while damage of cell membrane stability,resulting in membrane lipid peroxidation,increased relative conductivity and MDA content,and decreased SPAD value were observed under 30 ℃ or 35 ℃ for 2-4 d or 40 ℃ for 1-2 d.However,the damaged seedlings were still able to recover through increasing the content of soluble sugar and soluble protein,and raising the SOD,POD activities.Severe heat damages on the membrane system and antioxidant enzyme system indicated by sharply decreases in physiological indices were observed in the seedlings under 30,35 ℃ for 8 days or 40 ℃ for 4 days.When being treated at 40 ℃ for 8 days,95％ of the seedlings turned brown and died.Our studies demonstrated that the relative conductivity and MDA content were positively correlated,while the SPAD value was negatively correlated with temperatures.Therefore,these three values could be used as heat tolerance index of D.sinense seedlings.关键词
华石斛/热胁迫/形态变化/生理反应/耐热性Key words
Dendrobium sinense/heat stress/morphological change/physiological response/heat tolerance分类
农业科技引用本文复制引用
吴姝漪,孟千万,宋希强,丁琼,朱婕,余文刚,马豪治..高温胁迫对华石斛幼苗的形态和生理参数的影响[J].热带作物学报,2017,38(4):646-651,6.基金项目
国家农业公益性行业科研专项(No.201303117) （No.201303117）
中央财政林业科技推广示范资金(琼[2015] TG03) （琼[2015] TG03）
海南省应用技术研发与示范推广专项(No.ZDXM2015014). （No.ZDXM2015014）
