热带作物学报2017，Vol.38Issue(4)：652-658,7.DOI:10.3969/j.issn.1000-2561.2017.04.011
大苞鞘石斛组织培养快繁研究
Tissue Culture and Plant Regeneration of Dendrobium wardianum
摘要
Abstract
In order to study the effects of different factors on the tissue culture and plant regeneration of Dendrobium wardianum,three kinds of factors of three levels were used by an orthogonal design.The stem segments of D.wardianum were used as the explants for tissue culture.Results showed that the optimum PLBs induction medium was 1/2MS+2,4-D 0.2 mg/L+Sugar 30 g/L.When the shoot bud diameter reached to 1.5 mm,it must be detached and transferred to the same media for 15-20 days.The optimum medium for PLBs proliferation was 1/2MS+NAA 1 mg/L+Sugar 30 g/L+CW 10％ and the appropriate subculture cycle was about 25 to 30 days.The optimum PLBs differentiation medium was 1/2MS+NAA 0.5 mg/L+Sugar 30 g/L+CW 10％.The aseptic seedling which obtained from the former culture stage was cultured on the root induction media of Hyponex No.2 3 g/L+Peptone 2 g/L+IBA 1 mg/L+Sugar 30 g/L+CW 10％ for 45 days.The optimal medium of strong plantlets and rootage was Hyponex No.1 3 g/L+Peptone 2 g/L+Sugar 30 g/L+Activated carbon 1 g/L.关键词
大苞鞘石斛/组织培养/再生体系/拟原球茎Key words
Dendrobium wardianum/tissue culture/regeneration system/PLBs分类
农业科技引用本文复制引用
王伟,卢珊,葛冰,郜爱玲,金荷仙..大苞鞘石斛组织培养快繁研究[J].热带作物学报,2017,38(4):652-658,7.基金项目
浙江省教育厅高校国内访问工程师校企合作项目(No.FG2014199) （No.FG2014199）
浙江省高等教育课堂教学改革研究项目(No.kg2013979). （No.kg2013979）
