热带作物学报2017，Vol.38Issue(4)：689-694,6.DOI:10.3969/j.issn.1000-2561.2017.04.016
内生真菌HND5挥发性物质组分分析及其抑菌作用测定
Identification and Fungistasis of Volatile Compounds Produced by Endophytic Fungi HND5
摘要
Abstract
Endophytic fungi HND5 which was isolated from healthy leaves of Brachiaria sp.could inhibit many plant pathogens with volatile substances.In this article,solid phase micro-extraction (SPME) was used to collect volatile organic compounds (VOCs) produced by HND5 from the head space.And GC-MS was used to analyzed the volatile compounds of HND5.At last,14 compounds were identified from VOCs produced by HND5.Within these compounds,Caryophyllene and Benzene,4-ethenyl-1,2-dimethoxy-were found antagonistic against growth of Fusarium oxysporum,Colletotrichum gloeosporioides and Corynespora cassiicola.EC50 values of Caryophyllene were 2 916.07,729.54 and 404.23 μL/L,respectively.And EC50 values of Benzene,4-ethenyl-1,2-dimethoxy were 62.72,27.60 and 23.54 pL/L,respectively.关键词
内生真菌/挥发性有机物/气相色谱-质谱联用/抑菌作用Key words
endophyte/volatile organic compounds/GC-MS/fungistasis分类
农业科技引用本文复制引用
陈奕鹏,杨扬,时涛,蔡吉苗,黄贵修..内生真菌HND5挥发性物质组分分析及其抑菌作用测定[J].热带作物学报,2017,38(4):689-694,6.基金项目
农业部现代农业人才支撑计划项目(No.0316001) （No.0316001）
中央级公益性科研院所基本科研业务费(No.2016hzs1J020). （No.2016hzs1J020）
