热带作物学报2017，Vol.38Issue(4)：763-768,6.DOI:10.3969/j.issn.1000-2561.2017.04.028
微波辅助酶法制备米糠蛋白工艺对黄曲霉毒素B1脱除及酶解效果的影响
Effect of Microwave-assisted Enzyme Treatment on Aflatoxin B1 Removal and Enzymatic Hydrolysis During the Preparation of Rice Bran Protein
摘要
Abstract
In this study,the efficiency through the microwave-assisted enzyme treatment degradation of AFB1 in rice bran-protein was explored.Initial concentration of AFB1 was 102.54 μg/kg,and the conditions of the enzyme treatment were solid-liquid ratio (g:mL) 1:10,enzyme dosage 0.50％ of α-amylase and cellulase,pH 6.0,temperature 50 ℃ and treatment time 2 h.The results showed that the concentration of AFB1 decreased 45.36％ during the preparation of rice-bran protein by the enzyme treatment,and the residual concentration of AFB1 was 56.03 μg/kg,and the recovery rate of protein was 78.20％.Based on the enzyme treatment,after the treatment of microwave power per unit volume 750 W for 7 min,the degradation rate of AFB1 was 92.48％,and the residual amount of AFB1 was 4.21 μg/kg,which meets the requirement of national standard (≤ 10 μg/kg),and the recovery rate of protein was 81.36％.Immunoaffinity column clean-high performance liquid chromatography-fluorescence detection method could improve the detection limit of AFB1 to O.10 μg/kg.Microwave-assisted enzyme treatment contributed to the degradation of AFB1 in rice bran-protein and was beneficial to the recovery rate of protein.This method was simple and efficient,and it could be applied to the crops contaminated with aflatoxin.关键词
米糠蛋白/黄曲霉毒素B1/微波/脱除Key words
Rice bran protein/aflatoxin B1/microwave/removal分类
管理科学引用本文复制引用
林耀盛,陈智毅,张友胜,唐小俊,魏振承,张业辉,刘学铭..微波辅助酶法制备米糠蛋白工艺对黄曲霉毒素B1脱除及酶解效果的影响[J].热带作物学报,2017,38(4):763-768,6.基金项目
公益性行业(农业)科技专项(No.201203037). （农业）
