水产学报2017，Vol.41Issue(5)：649-657,9.DOI:10.11964/jfc.20160710479
凡纳滨对虾卵巢类围食膜蛋白基因克隆及其在早期胚胎发育中的表达
Cloning and expression analysis of shrimp ovarian peritrophin-like gene during early embryonic development in Litopenaeus vannamei
摘要
Abstract
Peritrophin was one type of proteins which was the important structural component of peritrophic membrane (PM) lining the midgut in invertebrate. It was first separated from insect, and played an important role in protecting insects from invasion by microorganisms. Recently, many peritrophin-like genes were identified from tissues not only intestine in crustacean, which implied that the role of peritrophin-like proteins might be diverse. In this study, two cDNA sequences encoding peritrophin-like protein named as LvPT1 and LvPT2 were found and cloned from Litopenaeus vannamei. Their deduced amino acid sequences exhibited high similarity to each other and most other shrimp peritrophins which have been reported. Tissue expression analysis by Real-time PCR revealed that LvPT1 and LvPT2 were mainly expressed in shrimp ovary, similar with shrimp ovarian peritrophins (SOPs). Semi-quantitative RT-PCR was used for analyzing the expression of LvPT2 during the early development of L. vannamei. The results showed that LvPT2 expression was the highest in fertilized eggs, and decreased significantly in developing process. There was no obvious expression of LvPT2 in nauplius hatched out from the hatching membrane. Therefore, we presumed thatLvPT1 and LvPT2 might be the same kind of peritrophin to play the similar role with other shrimp SOPs, in participating in the formation and protection of fertilized egg, and protecting shrimp embryo against pathogen infection.关键词
凡纳滨对虾/围食膜/围食膜蛋白/胚胎发育Key words
Litopenaeus vannamei/peritrophic membrane (PM)/peritrophin/embryonic development分类
生物科学引用本文复制引用
马欢,褚吉兴,王丽燕,李富花,相建海..凡纳滨对虾卵巢类围食膜蛋白基因克隆及其在早期胚胎发育中的表达[J].水产学报,2017,41(5):649-657,9.基金项目
国家自然科学基金(41076101) （41076101）
天津市"用三年时间引进千名以上高层次人才"项目(5KQM110029) （5KQM110029）
天津师范大学引进人才基金项目(5RL130)National Natural Science Foundation of China (41076101) （5RL130）
Foundation of Tianjin Project"With Three Years of Time to Introduce a Thousand or More High-level Talents" (5KQM110029) （5KQM110029）
Foundation of Talent Introduction Project of Tianjin Normal University (5RL130) （5RL130）
