水产学报2017，Vol.41Issue(5)：723-733,11.DOI:10.11964/jfc.20160610426
腹腔注射葡萄糖对大黄鱼血液生化指标及糖代谢关键酶表达量的影响
Effects of glucose injection on serum indices and gene expression of carbohydrate metabolic enzymes in large yellow croaker (Larimichthys crocea)
摘要
Abstract
This experiment was conducted to evaluate the effects of intraperitoneal glucose injection on serum indices, hepatic glycolysis and gluconeogenic key enzyme expression levels of intraperitoneal glucose injection of large yellow croaker (Larimichthys crocea). Fish [n=270, mean weight=(100±5) g] were randomly assigned to three groups, a control group that was injected with sterile 0.9% normal saline, the low dosage group that was injected with glucose (300 mg/kg wet weight, LG) and the high dosage group with glucose (1500 mg/kg wet weight, HG). The results showed that the plasma glucose level reached a peak at 3 h after both dose glucose injections, and returned to base level at the time of 24 h after injection of low dosage glucose, and it was still higher than base level after 24 h after high dosage glucose relatively. The trend of relative expression of HK gene after both dose glucose injections was increased first, then decreased, and reached the peak at 9 h. The relative expression of GK gene trend was to rise first and then remained unchanged and finally fell after both dose glucose injections, and it reached the peak at 9 h, but it was decreased at 4 h after high dosage glucose. The relative expression of PFK gene presented the trend of first increasing and then decreasing, and it reached the highest at 9 h after high dosage glucose. The trend of relative expression of PEPCK gene decreased first and then increased, and it reached the minimum at 2 h after high dosage glucose. It was significantly higher at 6 h than at any other time point, after high dosage glucose. These results indicate that the plasma glucose level of large yellow croaker increased, and maintained for a long time. The absence of inhibition in the expression of the gluconeogenic enzyme of G6Pase,FBPase concurrent with increased postprandia expression of GK led to postprandial hyperglycaemia in large yellow croaker.关键词
大黄鱼/腹腔注射/糖耐量/糖代谢关键酶/基因表达Key words
Larimichthys crocea/intraperitoneal injection/glucose tolerance/carbohydrate metabolic enzymes/gene expression分类
农业科技引用本文复制引用
李弋,邱红,侯迎梅,陆游,申屠基康,周歧存..腹腔注射葡萄糖对大黄鱼血液生化指标及糖代谢关键酶表达量的影响[J].水产学报,2017,41(5):723-733,11.基金项目
国家自然科学基金(31272670) （31272670）
宁波市农业科技攻关重大项目(2012C10025) （2012C10025）
浙江省2011协同创新项目(201101503) （201101503）
浙江省重中之重一级学科建设项目(20150108) （20150108）
岱衢洋大黄鱼专用饲料集成应用与示范(2014GA701001)National Natural Science Fundation of China (31272670) （2014GA701001）
Agricultural Research Projects in the Ningbo Science and Technology Department (2012C10025) （2012C10025）
2011 Cooperative Innovation Center of Zhejiang Province (201101503) （201101503）
The Construction of the Most Important Subjects in Zhejiang Province (20150108) （20150108）
Daiquyang Large Yellow Croaker Special Feed Integrated Application and Demonstration (2014GA701001) （2014GA701001）
