水产学报2017，Vol.41Issue(5)：785-794,10.DOI:10.11964/jfc.20160210291
高通量测序分析凡纳滨对虾育苗期水体菌群结构特征
Characterization of aquatic bacterial community of Litopenaeus vannamei larvae during hatchery period with high-throughput sequencing
摘要
Abstract
In order to characterize the bacterial community structure and diversity in rearing water of healthy Litopenaeus vannamei larvae during hatchery period, the genomic DNA of aquatic microbiota from larval stages of nauplius 5 (N5), zoea 2 (Z2), mysis 1 (M1) and postlarvae 1 (P1) was amplified with 16S rDNA V4 primers and sequenced by a high-throughput Illumina MiSeq platform. The results showed that the bacterial Shannon diversity index increased at P1 stage after declining gradually from stages N5 to M1, the Pielou evenness index at N5 stage was a little higher than those of the following three stages, which were approximated closely. During the entire larviculture period, the phylum Proteobacteria was overwhelmingly dominated in rearing water with high abundance up to 50.0%–88.0%, especially, the family Rhodobacteraceae of which had high abundances of 24.2%, 61.6%, 43.3%, and 51.8% at stages N5, Z2, M1, and P1, respectively. Bacteroidetes thrived in various stages with abundance higher than 9.7%, and the family Saprospiraceae of this phylum existed significantly (5.3%–16.9%) in the latter three phases. Actinobacteria presented also dominantly in rearing water except for the N5 stage, and the family Microbacteriaceae with abundances of 5.6%, 37.6% and 10.8% for Z2, M1, and P1 stages, respectively. The phylum Verrucomicrobia dominated only at P1 stage (5.3%). In the larviculture water, the core microbiota at genus level consisted ofLoktanella,Marivita,Donghicola,Sulfitobacter, and two unclassified candidates. Thus the microbiota in larval rearing water harbored a high diversity and presented dynamic succession, which differed obviously between N5 stage and the latter three stages. Overall, the main environmental factors in larviculture water varied slightly and exhibited no significant effect on abundance of dominant OTUs (operational taxonomic units). In conclusion, the bacterial community in rearing water of healthyL. vannamei larvae shifts in composition and abundance with larval development and diet feeding. And the core microbiota with potential functions play an important role in maintaining homeostasis in the larviculture system, which could serve as candidates for autochthonous probiotics, as well as healthy indicator for shrimp larviculture.关键词
凡纳滨对虾/水体菌群/育苗期/细菌多样性/高通量测序Key words
Litopenaeus vannamei/aquatic microbiota/hatchery phase/bacterial diversity/high-throughput sequencing分类
农业科技引用本文复制引用
薛明,何瑶瑶,邱孟德,梁华芳,陈第省,王立群,刘杨锋,温崇庆..高通量测序分析凡纳滨对虾育苗期水体菌群结构特征[J].水产学报,2017,41(5):785-794,10.基金项目
国家自然科学基金(31372536, 31502189) （31372536, 31502189）
广东省科技计划项目(2015A020209160)National Natural Science Foundation of China (31372536, 31502189) （2015A020209160）
Science and Technology Planning Project of Guangdong Province (2015A020209160) （2015A020209160）
