山东医药2017，Vol.57Issue(20)：9-12,4.DOI:10.3969/j.issn.1002-266X.2017.20.003
miR-106b在鼻咽癌组织中表达及对鼻咽癌CNE-2细胞生物学特性的影响
Expression of miR-106b in nasopharyngeal carcinoma tissues and its effects on the biological characteristics of nasopharyngeal carcinoma CNE-2 cells
摘要
Abstract
Objective To investigate the expression of miR-106b in nasopharyngeal carcinoma tissues and its effects on the biological characteristics of nasopharyngeal carcinoma CNE-2 cells.Methods Sixty-eight cases of surgical resection specimens from first treatment patients with nasopharyngeal carcinoma and 45 cases of nasopharyngeal biopsy specimens from patients with chronic nasopharyngitis were selected.The expression of miR-106b was detected by using real-time PCR technology.The relationship between the expression of miR-106b and clinicopathological parameters in patients with nasopharyngeal carcinoma was analyzed.The nasopharyngeal carcinoma CNE-2 cells in the logarithmic growth phase were divided into four groups (1×10.6 cells/group).Cells in the miR-106b mimics group and miR-106b inhibitor group were transected with miR-106b mimics and miR-106 inhibitor, respectively.Cells in the negative control group were transfected with negative control sequence, while cells in the blank control group were not treated.The cell proliferation of each transfected group was tested by using MTT assay.The cell cycle of each transfected group was detected by using flow cytometry.The cell migration and invasion of each transfected group were detected by using Transwell experiment.Results The relative expression levels of miR-106b in the nasopharyngeal carcinoma tissues and chronic nasopharyngitis nasopharyngeal tissues were 1.57±0.15 and 1.14±0.12, respectively, P<0.05.The expression of miR-106b in the nasopharyngeal carcinoma tissues was related with lymph node metastasis, carotid sheath violations, and skull base violations (all P<0.05).The relative expression level in each transfection group: the miR-106b mimic group was significantly higher than the miR-106b inhibitor group, the negative control group and blank control group, and the miR-106b inhibitor group was lower than the negative control group and blank control group, all P<0.05.The proliferation of each transfection group: A values after cell inoculation 24, 48, 72 and 96 h in the miR-106b mimic group were higher than those of the miR-106b inhibitor group, negative control group and blank control group, while the miR-106b inhibitor group was lower than the negative control group and blank control group, all P<0.05.The cell cycle of each transfection group: the proportion of S phase in the miR-106b mimics group was higher than that of the miR-106b inhibitor group, the negative control group and blank control group, while the miR-106b inhibitor group was lower than the negative control group and blank control group, all P<0.05.The cell migration and cell invasion in each transfection group: the number of migration cells and invasion cells in the miR-106b mimics group was higher than that of the miR-106b inhibitor group, negative control group and blank control group, and the miR-106b inhibitor group was lower than the negative control group and blank control group, all P<0.05.Conclusion miR-106b is highly expressed in nasopharyngeal carcinoma tissue, and the miR-106b overexpression may promote proliferation, migration, and invasion of nasopharyngeal carcinoma CNE-2 cells.关键词
鼻咽癌/微小RNA-106b/CNE-2细胞/细胞增殖/细胞迁移Key words
nasopharyngeal carcinoma/miR-106b/CNE-2 cells/cell proliferation/cell migration分类
医药卫生引用本文复制引用
周士霞,王海莉,王豪勋..miR-106b在鼻咽癌组织中表达及对鼻咽癌CNE-2细胞生物学特性的影响[J].山东医药,2017,57(20):9-12,4.基金项目
河南省科技发展计划(142102310087). （142102310087）
