山东医药2017，Vol.57Issue(20)：17-19,3.DOI:10.3969/j.issn.1002-266X.2017.20.005
瑞替加滨对离体大鼠十二指肠平滑肌收缩功能的影响及其机制
Effect of retigabine on contraction of smooth muscle of rat duodenum in vitro
摘要
Abstract
Objective To investigate the effect of retigabine on contraction of the smooth muscle of rat duodenum and to discuss its mechanism.Methods The rat duodenum was cut into segments with a length of about 1.5 cm.We used BL-420F Biological Function Experiment System for tension recording.After tension curve was stabilized, we performed the following experiments and each experiment was repeated 6 times.① We added 3.125, 6.25, 12.5, 25, 50, 100, 150, 200 μmol/L of retigabine into the solution.The recording time of each concentration was 15 min and each concentration was rinsed with Kreb's solution for 20 min.The mean amplitude of the smooth muscle tension of duodenum in vitro was measured before administration and after treatment with different concentrations of retigabine.② After 10 μmol/L XE-991 was added for 15 min, we added 20 μmol/L retigabine for 15 min.Then we compared the percent of mean amplitude of the smooth muscle tension of duodenum in vitro before and after retigabine.③ After 20 μmol/L retigabine was added for 15 min, we added 10 μmol/L XE-991 for 15 min.Then we compared the percent of mean amplitude of the smooth muscle tension of duodenum in vitro before and after XE-991.Results ① The percent of mean amplitude of the smooth muscle tension of duodenum in vitro before retigabine intervention was 100%.The percents of mean amplitude which were treated with retigabine were lower than that of the control group (0 μmol/L), P<0.05.In the range of 6.25-200 μmol/L, the percent of mean amplitude decreased gradually with the increase of retigabine concentration, P<0.05.② The percent of mean amplitude of smooth muscle tension which was incubated by XE-991 did not change significantly before and after retigabine intervention, P>0.05.③ The percent of mean amplitude of smooth muscle tension which was incubated by retigabine was higher than that after being given XE-991, P<0.05.Conclusion In the range of 6.25-200 μmol/L, retigabine can inhibit the contraction of rat duodenal smooth muscle in a dose-dependent manner, and this inhibitory effect may be due to the activation of potassium channel.关键词
瑞替加滨/十二指肠/平滑肌/收缩功能/钾离子通道/体外实验Key words
retigabine/duodenum/smooth muscle/contraction/potassium channel/in vitro experiment分类
医药卫生引用本文复制引用
高迪,陈渊锦,刘羽丹,张囡,沙磊..瑞替加滨对离体大鼠十二指肠平滑肌收缩功能的影响及其机制[J].山东医药,2017,57(20):17-19,3.基金项目
国家自然科学基金资助项目(81270900,81670492). （81270900,81670492）
