上海针灸杂志2017，Vol.36Issue(6)：744-750,7.DOI:10.13460/j.issn.1005-0957.2017.06.0744
电针对5月龄APP/PS1转基因小鼠海马组织及组织间液可溶性Aβ1-42水平的影响
Effect of Electroacupuncture on Hippocampal Tissue and Interstitial Fluid Soluble Aβ1-42 Levels in 5-month-old APP/PS1 Transgenic Mice
摘要
Abstract
Objective To investigate the intervening effect of electroacupuncture on brain interstitial fluid (ISF) and hippocampal soluble Aβ1-42 levels in mice with early Alzheimer's disease.Method Sixteen 5-month-old male APP/PS1 positive transgenic mice were randomized to model and acupuncture groups, 8 mice each in one cage. Isosexual negative transgenic mice constituted a normal control group. Electroacupuncture at Baihui(GV20) and Yongquan(KI1) or binding under the same condition was used as an intervention measure. Mouse hippocampal interstitial fluid was obtained by brain microdialysis. Hippocampal tissue and ISFβ amyloid protein 1-42 levels were measured by ELISA in the three groups of mice at 6, 7, 8 and 9 hrs after intervention to observe the changes. Result Hippocampal tissue and ISF soluble Aβ1-42 levels were significantly higher in the model group than in the normal group (P<0.01). ISF soluble Aβ1-42 levels were significantly lower in the acupuncture group of mice than in the model group (P<0.05) and there were no statistically significant differences in hippocampal Aβ1-42 levels between the two groups (P>0.05). The results of sample detection did not show a tendency to change over time (P>0.05). Conclusion Electroacupuncture can decrease hippocampal soluble Aβ1-42 levels in a mouse model of AD.关键词
阿尔茨海默病/电针/早期干预/脑组织间液/微透析/小鼠Key words
Alzheimer's disease/Electroacupuncture/Early intervention/Brain interstitial fluid/Microdialysis/Mice分类
医药卫生引用本文复制引用
步青云,薛卫国..电针对5月龄APP/PS1转基因小鼠海马组织及组织间液可溶性Aβ1-42水平的影响[J].上海针灸杂志,2017,36(6):744-750,7.基金项目
国家自然科学基金项目(81273826) （81273826）
北京中医药大学自主选题项目(2015-JYB-XS120) （2015-JYB-XS120）
