色谱2017，Vol.35Issue(6)：565-571,7.DOI:10.3724/SP.J.1123.2017.03018
模板法制备大孔硅胶微球及其在高效液相色谱蛋白质分离中的应用
Preparation of large-pore silica microspheres using templating method and their applications to protein separation with high performance liquid chromatography
摘要
Abstract
Large-pore silica microspheres were synthesized by utilizing weak cation exchange polymer beads as templates,N-trimethoxysilylpropyl-N,N,N-trimethylammonium chloride (TMSPTMA) as a structure-directing agent,tetraethoxysilane (TEOS) as a silica precursor,and triethanolamine as a weak base catalyst.The hydrolysis and condensation of the silica precursors occurred inside the templating polymer beads yielded polymer/silica composite microspheres.After the organic polymer templates were removed in the calcination step,large-pore silica microspheres were produced.The effects of different reaction conditions on the morphology,structure and dispersibility of the formed silica microspheres were investigated.It has been shown that when the volume ratio of TMSPTMA,TEOS and triethanolamine was 1:2:2,silica microspheres with pore size range of 50-150 nm and particle size around 2 μm were obtained.The as-prepared silica microspheres were then bonded with chlorodimethyloctadecylsilane (C18),packed into a 50 mm×4.6 mm column,and evaluated for the separations of some common standard proteins and soybean isolation proteins.The results showed that the large-pore silica spheres from this work have potentials for protein separation in HPLC.关键词
模板法/结构导向剂/大孔二氧化硅微球/高效液相色谱/蛋白质分离Key words
templating method/structure-directing agent/large-pore silica microspheres/high performance liquid chromatography (HPLC)/protein separation分类
化学化工引用本文复制引用
牛梦娜,马红彦,胡飞,王世革,刘璐,常海洲,黄明贤..模板法制备大孔硅胶微球及其在高效液相色谱蛋白质分离中的应用[J].色谱,2017,35(6):565-571,7.基金项目
上海市科委项目(14440502300) （14440502300）
上海理工大学沪江基金(D15011).Science and Technology Commission of Shanghai Municipality Project (No.14440502300) （D15011）
Foundation of Hujiang from University of Shanghai for Science and Technology (No.D15011). （No.D15011）
