色谱2017，Vol.35Issue(6)：601-607,7.DOI:10.3724/SP.J.1123.2016.12028
同位素内标-液相色谱-串联质谱法同时测定粮食及其制品中的5种真菌毒素
Simultaneous determination of five mycotoxins in various grains and their products by liquid chromatography-tandem mass spectrometry with isotope internal standard
高蓓 1姜德铭 1杨永坛1
作者信息
- 1. 中粮营养健康研究院,营养健康与食品安全北京市重点实验室,北京 102209
- 折叠
摘要
Abstract
A rapid method was established for the determination of zearalenone (ZON),nivalenol (NIV),deoxynivalenol (DON),3-acetyl-deoxynivalenol (3-ACDON) and 15-acetyl-deoxynivalenol (15-ACDON) in various grains and their products by liquid chromatography-tandem mass spectrometry (LC-MS/MS).The samples were extracted by acetonitrile-water (84:16,v/v),and cleaned-up by multifunctional column without any further step.The separation was carried out on an Acquity UPLC? BEH C18 column (100 mm×2.1 mm,1.7 μm) with gradient elution using acetonitrile-water as mobile phases.The analytes were quantified by isotope internal standard method.The correlation coefficients (r.2) were all above 0.99.The limits of detection (LODs,S/N=3) ranged from 5 μg/kg to 20 μg/kg.The recoveries of the five mycotoxins spiked in blank grain and its product samples varied from 84.2% to 114.5%,with the relative standard deviations (RSDs) of 0.4%-9.9% (n=6).The method is accurate,efficient,sensitive and practical.关键词
液相色谱-串联质谱法/同位素内标/真菌毒素/粮食及其制品Key words
liquid chromatography-tandem mass spectrometry (LC-MS/MS)/isotope internal standard/mycotoxins/grains and their products分类
化学化工引用本文复制引用
高蓓,姜德铭,杨永坛..同位素内标-液相色谱-串联质谱法同时测定粮食及其制品中的5种真菌毒素[J].色谱,2017,35(6):601-607,7.
