色谱2017，Vol.35Issue(6)：613-619,7.DOI:10.3724/SP.J.1123.2017.02008
在线液相色谱-二极管阵列检测器-串联质谱法检测野生菌中鹅膏毒肽和鬼笔毒肽
Determination of amanitins and phallotoxins in wild mushrooms by online liquid chromatography-diode array detector-tandem mass spectrometry
摘要
Abstract
A fast and wide linear range method was established for the determination of mushroom toxins amanitins (α-amanitin,β-amanitin and γ-amanitin) and phallotoxins (phallacidin and phalloidin) in wild mushrooms by online liquid chromatography-diode array detector-tandem mass spectrometry (LC-DAD-MS/MS).The mushroom toxins were extracted with methanol,and diluted with water.The extracts were separated on an XBridgeTM BEH C18 column (150 mm×3.0 mm,2.5 μm) under pH 10.7,measured by DAD and then analyzed by MS/MS.Basic mobile phase conditions were applied to improve the ionization efficiency of hydrogen ion adducts.The baseline separation of the analytes was obtained within 15 min.The limits of detection (LODs) of the sample matrix were 0.005-0.02 mg/kg.The toxins were quantified by the results measured by MS/MS when the toxin contents less than 2 mg/kg,and quantified by the results obtained from DAD when the contents more than 2 mg/kg.The linear range was 0.05-500 mg/kg for the whole method in one injection.The method was successfully applied to the analyses of amanitins and phallotoxins in Lepiota brunneoincarnata and white Amanita.关键词
在线液相色谱-二极管阵列检测器-串联质谱法/宽线性范围定量/蘑菇毒素/鹅膏毒肽/鬼笔毒肽/野生菌Key words
online liquid chromatography-diode array detector-tandem mass spectrometry (LC-DAD-MS/MS)/wide linear range quantification/mushroom toxins/amanitins/phallotoxins/wild mushrooms分类
化学化工引用本文复制引用
徐小民,张京顺,蔡增轩,张晶,黄百芬,许娇娇,陈苘..在线液相色谱-二极管阵列检测器-串联质谱法检测野生菌中鹅膏毒肽和鬼笔毒肽[J].色谱,2017,35(6):613-619,7.基金项目
浙江省医药卫生科技计划项目(2015DTA006).Science Research Foundation Program of Zhejiang Provincial Medicine and Health (No.2015DTA006). （2015DTA006）
