色谱2017，Vol.35Issue(6)：627-633,7.DOI:10.3724/SP.J.1123.2017.02010
超高效液相色谱-串联质谱法同时测定不同饲料中30种真菌毒素
Simultaneous determination of 30 mycotoxins in different feed products by ultra high performance liquid chromatography-tandem mass spectrometry
摘要
Abstract
A method for the simultaneous determination of 30 mycotoxins in different feed products was developed using a QuEChERS pretreatment procedure coupled with ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS).The samples were extracted with 5 mL water and 5 mL acetonitrile containing 1% (v/v) formic acid.An aliquot of the supernatant was dried by nitrogen gas,re-dissolved by 1mL water containing 5 mmol/L ammonium acetate-acetonitrile (80:20,v/v),and analyzed by UHPLC-MS/MS.Matrix-matched calibration curves and internal standards were used for accurate quantification.Satisfactory recoveries at low,medium and high spiked levels were ranged from 72.0% to 118.4% (n=5).Good linear relationships were obtained,the correlation coefficients (r2) were greater than 0.99.The limits of detection (LODs,S/N=3) and the limits of quantification (LOQs,S/N=10) ranged from 0.7 μg/L to 20 μg/L and from 2 μg/L to 50 μg/L,respectively.The proposed method is simple,rapid and valuable,which can be a powerful tool for quantitative analysis of the 30 mycotoxins in premixed feed,concentrated feed and formula feed products.关键词
超高效液相色谱-串联质谱/QuEChERS/真菌毒素/饲料Key words
ultra high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)/QuEChERS/mycotoxins/feed分类
化学化工引用本文复制引用
范志辰,韩铮,郭文博,赵志辉..超高效液相色谱-串联质谱法同时测定不同饲料中30种真菌毒素[J].色谱,2017,35(6):627-633,7.基金项目
沪农科攻字(2014)第3-2号(G20140302).Shanghai Agriculture Applied Technology Development Program of China (No.G20140302). （2014）
